Skip to main content

Research Repository

Advanced Search

Enaptin, a giant actin-binding protein, is an element of the nuclear membrane and the actin cytoskeleton

Padmakumar, V.C.; Abraham, S.; Braune, S.; Noegel, A.A.; Tunggal, B.; Karakesisoglou, I.; Korenbaum, E.

Authors

V.C. Padmakumar

S. Abraham

S. Braune

A.A. Noegel

B. Tunggal

E. Korenbaum



Abstract

Enaptin belongs to a family of recently identified giant proteins that associate with the F-actin cytoskeleton as well as the nuclear membrane. It is composed of an N-terminal alpha-actinin type actin-binding domain (ABD) followed by a long coiled coil rod and a transmembrane domain at the C-terminus. The ABD binds to F-actin in vivo and in vitro and leads to bundle formation. The human Enaptin gene spreads over 515 kb and gives rise to several splicing isoforms (Nesprin-1, Myne-1, Syne-1, CPG2). The longest assembled cDNA encompasses 27,669 bp and predicts a 1014 kDa protein. Antibodies against the ABD of Enaptin localise the protein at F-actin-rich structures throughout the cell and in focal contacts as well as at the nuclear envelope. In COS7 cells, the protein is also present within the nuclear compartment. With the discovery of the actin-binding properties of Enaptin and the highly homologous Nuance, we define a family of proteins that integrate the cytoskeleton with the nucleoskeleton.

Citation

Padmakumar, V., Abraham, S., Braune, S., Noegel, A., Tunggal, B., Karakesisoglou, I., & Korenbaum, E. (2004). Enaptin, a giant actin-binding protein, is an element of the nuclear membrane and the actin cytoskeleton. Experimental Cell Research, 295(2), 330-9

Journal Article Type Article
Publication Date 2004
Journal Exp Cell Res
Print ISSN 0014-4827
Publisher Elsevier
Volume 295
Issue 2
Pages 330-9
Public URL https://durham-repository.worktribe.com/output/1557595
Publisher URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15093733