Synthesis of 5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate and its Enzymatic Incorporation at the 5'-Termini of RNA Molecules

Williamson, D.; Cann, M.J.; Hodgson, D.R.W.

Abstract

5-Amino-5-deoxyguanosine-5-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5-H2N-RNA that can be conjugated to activated esters.

Citation

Williamson, D., Cann, M., & Hodgson, D. (2007). Synthesis of 5'-Amino-5'-deoxyguanosine-5'-N-phosphoramidate and its Enzymatic Incorporation at the 5'-Termini of RNA Molecules. Chemical Communications, 2007(47), 5096-5098. https://doi.org/10.1039/b712066d

• Journal Article Type: Article
• Acceptance Date: Sep 19, 2007
• Online Publication Date: Oct 11, 2007
• Publication Date: Oct 11, 2007
• Deposit Date: Jan 18, 2008
• Journal: Chemical Communications
• Print ISSN: 1359-7345
• Electronic ISSN: 1364-548X
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 2007
• Issue: 47
• Pages: 5096-5098
• DOI: https://doi.org/10.1039/b712066d
• Public URL: https://durham-repository.worktribe.com/output/1557490