DNA binding studies of cationic lanthanide complexes bearing a phenanthridinium group

Bobba, G.; Kean, S.D.; Parker, D.; Beeby, A.; Baker, G.

Abstract

The binding of tetracationic Lambda- and Delta -europium complexes of tetraamide and triamide ligands incorporating an N-methylphenanthridinium group to calf-thymus DNA, poly(dGdC) and super-coiled plasmid DNA has been monitored by absorption, luminescence and CD difference spectroscopy. With the tetraamide complexes, preferential binding to (CG) base-pairs was signalled by pronounced hypochromism, a small red shift and substantial fluorescence quenching with a limiting base-pair complex ratio of 2:1, consistent with an intercalative interaction and charge neutralisation. For the triamide Eu complex; a lower base-pair : complex ratio was found and changes in the form and polarisation of the Eu emission spectrum were consistent with coordination of a nucleobase phosphate group to the hydrated Eu centre.

Citation

Bobba, G., Kean, S., Parker, D., Beeby, A., & Baker, G. (2001). DNA binding studies of cationic lanthanide complexes bearing a phenanthridinium group. Journal of the Chemical Society-Perkin Transactions 2, 2001(9), 1738-1741. https://doi.org/10.1039/B104795G

• Journal Article Type: Article
• Acceptance Date: Jul 12, 2001
• Online Publication Date: Aug 16, 2001
• Publication Date: Aug 16, 2001
• Journal: Journal of the Chemical Society, Perkin Transactions 2
• Print ISSN: 1470-1820
• Electronic ISSN: 2050-8247
• Peer Reviewed: Peer Reviewed
• Volume: 2001
• Issue: 9
• Pages: 1738-1741
• DOI: https://doi.org/10.1039/B104795G
• Public URL: https://durham-repository.worktribe.com/output/1555615