Regulation of prokaryotic adenylyl cyclases by CO₂

Hammer, A.; Hodgson, D.R.W.; Cann, M.J.

Abstract

The Slr1991 adenylyl cyclase of the model prokaroyte Synechocystis PCC 6803 was stimulated 2-fold at 20 mM total Ci (inorganic carbon) atpH 7.5 through an increase in kcat.Adose response demonstrated an EC50 of 52.7 mMtotal Ci at pH 6.5. Slr1991 adenylyl cyclase was activated by CO2, but not by HCO3−. CO2 regulation of adenylyl cyclase was conserved in the CyaB1 adenylyl cyclase of Anabaena PCC 7120. These adenylyl cyclases represent the only identified signalling enzymes directly activated by CO2. The findings prompt an urgent reassessment of the activating carbon species for proposed HCO3−-activated adenylyl cyclases.

Citation

Hammer, A., Hodgson, D., & Cann, M. (2006). Regulation of prokaryotic adenylyl cyclases by CO₂. Biochemical Journal, 396(2), 215-218. https://doi.org/10.1042/bj20060372

• Journal Article Type: Article
• Publication Date: Jan 1, 2006
• Deposit Date: May 10, 2007
• Publicly Available Date: Nov 27, 2014
• Journal: Biochemical Journal
• Print ISSN: 0264-6021
• Electronic ISSN: 1470-8728
• Publisher: Portland Press
• Peer Reviewed: Peer Reviewed
• Volume: 396
• Issue: 2
• Pages: 215-218
• DOI: https://doi.org/10.1042/bj20060372
• Keywords: Adenylyl cyclase (adenyl cyclase, Adenylate; cyclase), Bicarbonate (HCO3−), cAMP, Carbon dioxide (CO2),; Synechocystis.
• Public URL: https://durham-repository.worktribe.com/output/1551662