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ACF7: An essential integrator of microtubule dynamics

Kodama, A.; Karakesisoglou, I.; Wong, E.; Vaezi, A.; Fuchs, E.


A. Kodama

E. Wong

A. Vaezi

E. Fuchs


ACF7 is a member of the spectraplakin family of cytoskeletal crosslinking proteins possessing actin and microtubule binding domains. Here, we show that ACF7 is an essential integrator of MT-actin dynamics. In endodermal cells, ACF7 binds along microtubules but concentrates at their distal ends and at cell borders when polarized. In ACF7's absence, microtubules still bind EB1 and CLIP170, but they no longer grow along polarized actin bundles, nor do they pause and tether to actin-rich cortical sites. The consequences are less stable, long microtubules with skewed cytoplasmic trajectories and altered dynamic instability. In response to wounding, ACF7 null cultures activate polarizing signals, but fail to maintain them and coordinate migration. Rescue of these defects requires ACF7's actin and microtubule binding domains. Thus, spectraplakins are important for controlling microtubule dynamics and reinforcing links between microtubules and polarized F-actin, so that cellular polarization and coordinated cell movements can be sustained.


Kodama, A., Karakesisoglou, I., Wong, E., Vaezi, A., & Fuchs, E. (2003). ACF7: An essential integrator of microtubule dynamics. Cell, 115(3), 343-354.

Journal Article Type Article
Publication Date Oct 1, 2003
Deposit Date Jan 17, 2008
Journal Cell
Print ISSN 0092-8674
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 115
Issue 3
Pages 343-354