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Nesprin interchain associations control nuclear size.

Lu, W.; Schneider, M.; Neumann, S.; Jaeger, V-M.; Taranum, S.; Munck, M.; Cartwright, S.; Richardson, C.; Carthew, J.; Noh, K.; Goldberg, M.; Noegel, A.; Karakesisoglou, I.


W. Lu

M. Schneider

S. Neumann

V-M. Jaeger

S. Taranum

M. Munck

S. Cartwright

C. Richardson

J. Carthew

K. Noh

A. Noegel


Nesprins-1/-2/-3/-4 are nuclear envelope proteins, which connect nuclei to the cytoskeleton. The largest nesprin-1/-2 isoforms (termed giant) tether F-actin through their N-terminal actin binding domain (ABD). Nesprin-3, however, lacks an ABD and associates instead to plectin, which binds intermediate filaments. Nesprins are integrated into the outer nuclear membrane via their C-terminal KASH-domain. Here, we show that nesprin-1/-2 ABDs physically and functionally interact with nesprin-3. Thus, both ends of nesprin-1/-2 giant are integrated at the nuclear surface: via the C-terminal KASH-domain and the N-terminal ABD-nesprin-3 association. Interestingly, nesprin-2 ABD or KASH-domain overexpression leads to increased nuclear areas. Conversely, nesprin-2 mini (contains the ABD and KASH-domain but lacks the massive nesprin-2 giant rod segment) expression yields smaller nuclei. Nuclear shrinkage is further enhanced upon nesprin-3 co-expression or microfilament depolymerization. Our findings suggest that multivariate intermolecular nesprin interactions with the cytoskeleton form a lattice-like filamentous network covering the outer nuclear membrane, which determines nuclear size.


Lu, W., Schneider, M., Neumann, S., Jaeger, V., Taranum, S., Munck, M., …Karakesisoglou, I. (2012). Nesprin interchain associations control nuclear size. Cellular and Molecular Life Sciences, 69(20), 3493-3509.

Journal Article Type Article
Publication Date 2012-10
Deposit Date May 14, 2012
Journal Cellular and Molecular Life Sciences
Print ISSN 1420-682X
Electronic ISSN 1420-9071
Publisher Springer
Peer Reviewed Peer Reviewed
Volume 69
Issue 20
Pages 3493-3509
Keywords Actin binding domain, Cytoskeleton, KASH-domain, LINC complex, Nesprin, Nuclear envelope, Nuclear shape, SUN-domain