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The utility of yeast as a tool for cell-based, target-directed high-throughput screening

Norcliffe, J.L.; Alvarez-Ruiz, E.; Martin-Plaza, J.J.; Steel, P.G.; Denny, P.W.

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Authors

J.L. Norcliffe

E. Alvarez-Ruiz

J.J. Martin-Plaza

P.G. Steel



Abstract

Many Neglected Tropical Diseases (NTDs) have recently been subject of increased focus, particularly with relation to high-throughput screening (HTS) initiatives. These vital endeavours largely rely of two approaches, in vitro target-directed screening using biochemical assays or cell-based screening which takes no account of the target or targets being hit. Despite their successes both of these approaches have limitations; for example, the production of soluble protein and a lack of cellular context or the problems and expense of parasite cell culture. In addition, both can be challenging to miniaturize for ultra (u)HTS and expensive to utilize. Yeast-based systems offer a cost-effective approach to study and screen protein targets in a direct-directed manner within a eukaryotic cellular context. In this review, we examine the utility and limitations of yeast cell-based, target-directed screening. In particular we focus on the currently under-explored possibility of using such formats in uHTS screening campaigns for NTDs.

Citation

Norcliffe, J., Alvarez-Ruiz, E., Martin-Plaza, J., Steel, P., & Denny, P. (2014). The utility of yeast as a tool for cell-based, target-directed high-throughput screening. Parasitology, 141(1), 8-16. https://doi.org/10.1017/s0031182013000425

Journal Article Type Article
Publication Date Jan 1, 2014
Deposit Date Apr 25, 2013
Publicly Available Date Jan 15, 2014
Journal Parasitology
Print ISSN 0031-1820
Electronic ISSN 1469-8161
Publisher Cambridge University Press
Peer Reviewed Peer Reviewed
Volume 141
Issue 1
Pages 8-16
DOI https://doi.org/10.1017/s0031182013000425
Keywords Yeast, HTS, NTDs.

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