The utility of yeast as a tool for cell-based, target-directed high-throughput screening

Norcliffe, J.L.; Alvarez-Ruiz, E.; Martin-Plaza, J.J.; Steel, P.G.; Denny, P.W.

Abstract

Many Neglected Tropical Diseases (NTDs) have recently been subject of increased focus, particularly with relation to high-throughput screening (HTS) initiatives. These vital endeavours largely rely of two approaches, in vitro target-directed screening using biochemical assays or cell-based screening which takes no account of the target or targets being hit. Despite their successes both of these approaches have limitations; for example, the production of soluble protein and a lack of cellular context or the problems and expense of parasite cell culture. In addition, both can be challenging to miniaturize for ultra (u)HTS and expensive to utilize. Yeast-based systems offer a cost-effective approach to study and screen protein targets in a direct-directed manner within a eukaryotic cellular context. In this review, we examine the utility and limitations of yeast cell-based, target-directed screening. In particular we focus on the currently under-explored possibility of using such formats in uHTS screening campaigns for NTDs.

Citation

Norcliffe, J., Alvarez-Ruiz, E., Martin-Plaza, J., Steel, P., & Denny, P. (2014). The utility of yeast as a tool for cell-based, target-directed high-throughput screening. Parasitology, 141(1), 8-16. https://doi.org/10.1017/s0031182013000425

• Journal Article Type: Article
• Publication Date: Jan 1, 2014
• Deposit Date: Apr 25, 2013
• Publicly Available Date: Jan 15, 2014
• Journal: Parasitology
• Print ISSN: 0031-1820
• Electronic ISSN: 1469-8161
• Publisher: Cambridge University Press
• Peer Reviewed: Peer Reviewed
• Volume: 141
• Issue: 1
• Pages: 8-16
• DOI: https://doi.org/10.1017/s0031182013000425
• Keywords: Yeast, HTS, NTDs.
• Public URL: https://durham-repository.worktribe.com/output/1478097