Skip to main content

Research Repository

Advanced Search

Embryonic and adult isoforms of XLAP2 form microdomains associated with chromatin and the nuclear envelope

Chmielewska, M; Dubińska-Magiera, M; Sopel, M; Rzepecka, D; Hutchison, CJ; Goldberg, MW; Rzepecki, R.

Embryonic and adult isoforms of XLAP2 form microdomains associated with chromatin and the nuclear envelope Thumbnail


Authors

M Chmielewska

M Dubińska-Magiera

M Sopel

D Rzepecka

CJ Hutchison

R. Rzepecki



Abstract

Laminin-associated polypeptide 2 (LAP2) proteins are alternatively spliced products of a single gene; they belong to the LEM domain family and, in mammals, locate to the nuclear envelope (NE) and nuclear lamina. Isoforms lacking the transmembrane domain also locate to the nucleoplasm. We used new specific antibodies against the N-terminal domain of Xenopus LAP2 to perform immunoprecipitation, identification and localization studies during Xenopus development. By immunoprecipitation and mass spectrometry (LC/MS/MS), we identified the embryonic isoform XLAP2γ, which was downregulated during development similarly to XLAP2ω. Embryonic isoforms XLAP2ω and XLAP2γ were located in close association with chromatin up to the blastula stage. Later in development, both embryonic isoforms and the adult isoform XLAP2β were localized in a similar way at the NE. All isoforms colocalized with lamin B2/B3 during development, whereas XLAP2β was colocalized with lamin B2 and apparently with the F/G repeat nucleoporins throughout the cell cycle in adult tissues and culture cells. XLAP2β was localized in clusters on chromatin, both at the NE and inside the nucleus. Embryonic isoforms were also localized in clusters at the NE of oocytes. Our results suggest that XLAP2 isoforms participate in the maintenance and anchoring of chromatin domains to the NE and in the formation of lamin B microdomains.

Citation

Chmielewska, M., Dubińska-Magiera, M., Sopel, M., Rzepecka, D., Hutchison, C., Goldberg, M., & Rzepecki, R. (2011). Embryonic and adult isoforms of XLAP2 form microdomains associated with chromatin and the nuclear envelope. Cell and Tissue Research, 344(1), 97-110. https://doi.org/10.1007/s00441-011-1129-2

Journal Article Type Article
Publication Date Apr 1, 2011
Deposit Date Apr 30, 2012
Publicly Available Date Jun 12, 2012
Journal Cell and Tissue Research
Print ISSN 0302-766X
Electronic ISSN 1432-0878
Publisher Springer
Peer Reviewed Peer Reviewed
Volume 344
Issue 1
Pages 97-110
DOI https://doi.org/10.1007/s00441-011-1129-2
Keywords XLAP2, Nuclear envelope, Nuclear lamina, Chromatin, Cell cycle, Development, Xenopus laevis (Anura)

Files

Published Journal Article (1.1 Mb)
PDF

Copyright Statement
This article is published under a Creative Commons Attribution (CC-BY) license.







You might also like



Downloadable Citations