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A Superfamily of Actin-Binding Proteins
at the Actin-Membrane Nexus of Higher Plants.

Deeks, Michael J.; Calcutt, Joanna R.; Ingle, Elizabeth K.S.; Hawkins, Timothy J.; Chapman, Sean; Richardson, A.Christine; Mentlak, David A.; Dixon, Martin R.; Cartwright, Frances; Smertenko, Andrei P.; Oparka, Karl; Hussey, Patrick J.

Authors

Michael J. Deeks

Joanna R. Calcutt

Elizabeth K.S. Ingle

Sean Chapman

A.Christine Richardson

David A. Mentlak

Martin R. Dixon

Frances Cartwright

Andrei P. Smertenko

Karl Oparka



Abstract

Complex animals use a wide variety of adaptor proteins to produce specialized sites of interaction between actin and membranes. Plants do not have these protein families, yet actin-membrane interactions within plant cells are critical for the positioning of subcellular compartments, for coordinating intercellular communication, and for membrane deformation [1]. Novel factors are therefore likely to provide interfaces at actin-membrane contacts in plants, but their identity has remained obscure. Here we identify the plant-specific Networked (NET) superfamily of actin-binding proteins, members of which localize to the actin cytoskeleton and specify different membrane compartments. The founding member of the NET superfamily, NET1A, is anchored at the plasma membrane and predominates at cell junctions, the plasmodesmata. NET1A binds directly to actin filaments via a novel actin-binding domain that defines a superfamily of thirteen Arabidopsis proteins divided into four distinct phylogenetic clades. Members of other clades identify interactions at the tonoplast, nuclear membrane, and pollen tube plasma membrane, emphasizing the role of this superfamily in mediating actin-membrane interactions.

Journal Article Type Article
Publication Date 2012-09
Deposit Date Aug 10, 2012
Journal Current Biology
Print ISSN 0960-9822
Publisher Cell Press
Peer Reviewed Peer Reviewed
Volume 22
Issue 17
Pages 1595-1600
DOI https://doi.org/10.1016/j.cub.2012.06.041
Public URL https://durham-repository.worktribe.com/output/1474369