Professor Junjie Wu junjie.wu@durham.ac.uk
Professor
Professor Junjie Wu junjie.wu@durham.ac.uk
Professor
F.M. Tholozan
Professor Martin Goldberg m.w.goldberg@durham.ac.uk
Professor
Leon Bowen leon.bowen@durham.ac.uk
Senior Manager (Electron Microscopy)
Professor Junjie Wu junjie.wu@durham.ac.uk
Professor
Professor Roy Quinlan r.a.quinlan@durham.ac.uk
Emeritus Professor
Fibroblast growth factors play a key role in regulating lens epithelial cell proliferation and differentiation via an anteroposterior gradient that exists between the aqueous and vitreous humours. FGF-2 is the most important for lens epithelial cell proliferation and differentiation. It has been proposed that the presentation of FGF-2 to the lens epithelial cells involves the lens capsule as a source of matrix-bound FGF-2. Here we used immunogold labelling to measure the matrix-bound FGF-2 gradient on the inner surface of the lens capsule in flat-mounted preparations to visualize the FGF-2 available to lens epithelial cells. We also correlated FGF-2 levels with levels of its matrix-binding partner perlecan, a heparan sulphate proteoglycan (HSPG) and found the levels of both to be highest at the lens equator. These also coincided with increased levels of phosphorylated extracellular signal-regulated kinase 1 and 2 (pERK1/2) in lens epithelial cells that localised to condensed chromosomes of epithelial cells that were Ki-67 positive. The gradient of matrix-bound FGF-2 (anterior pole: 3.7 ± 1.3 particles/μm2; equator: 8.2 ± 1.9 particles/μm2; posterior pole: 4 ± 0.9 particles/μm2) and perlecan (anterior pole: 2.1 ± 0.4 particles/μm2; equator: 5 ± 2 particles/μm2; posterior pole: 1.9 ± 0.7 particles/μm2) available at the inner lens capsule surface was measured for the bovine lens. These data support the anteroposterior gradient hypothesis and provide the first measurement of the gradient for an important morphogen and its HSPG partner, perlecan, at the epithelial cell-lens capsule interface.
Wu, W., Tholozan, F., Goldberg, M., Bowen, L., Wu, J., & Quinlan, R. (2014). A gradient of matrix-bound FGF-2 and perlecan is available to lens epithelial cells. Experimental Eye Research, 120, 10-14. https://doi.org/10.1016/j.exer.2013.12.004
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 30, 2014 |
Publication Date | Mar 1, 2014 |
Deposit Date | May 1, 2014 |
Publicly Available Date | May 6, 2014 |
Journal | Experimental Eye Research |
Print ISSN | 0014-4835 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 120 |
Pages | 10-14 |
DOI | https://doi.org/10.1016/j.exer.2013.12.004 |
Keywords | Lens capsule, FGF-2, Perlecan, ERK1/2. |
Public URL | https://durham-repository.worktribe.com/output/1465969 |
Published Journal Article
(2.2 Mb)
PDF
Copyright Statement
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
Biotribological and Structural Analyses of Large Diameter Ceramic on Carbon Fibre Reinforced PEEK Hip Joints with Different Inclination
(2011)
Presentation / Conference Contribution
A mathematical model of the eye lens epithelium of mammals that predicts cell density profiles in the ageing lens.
(2015)
Presentation / Conference Contribution
Prosim Simulator Wear Study of 60mm Diameter Zirconia Toughened Alumina Composite Hip Bearings: The Effect of High Cup Inclination Angle and Microseparation
(2011)
Presentation / Conference Contribution
Effect of Knee, Ankle and Hip Joint Replacements on Vitamin E Infused Highly Crosslinked UHMWPE Wear Particles Size, Shape and Morphologies Using a New Modified Base Digestion Method
(2015)
Presentation / Conference Contribution
Do structural alterations mediated by enzymatic activities influence the mechanical properties of capsular biomembranes?
(2014)
Presentation / Conference Contribution
About Durham Research Online (DRO)
Administrator e-mail: dro.admin@durham.ac.uk
This application uses the following open-source libraries:
Apache License Version 2.0 (http://www.apache.org/licenses/)
Apache License Version 2.0 (http://www.apache.org/licenses/)
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search