Cyril Bourgenot cyril.bourgenot@durham.ac.uk
Associate Professor
3D adaptive optics in a light sheet microscope
Bourgenot, Cyril; Saunter, Christopher D.; Taylor, Jonathan M.; Girkin, John M.; Love, Gordon D.
Authors
Christopher D. Saunter
Jonathan M. Taylor
Professor John Girkin j.m.girkin@durham.ac.uk
Professor
Gordon D. Love
Abstract
We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish. We show improvements in image quality whilst recording a 3D “z–stack” and show how the aberrations come from varying depths in the fish.
Citation
Bourgenot, C., Saunter, C. D., Taylor, J. M., Girkin, J. M., & Love, G. D. (2012). 3D adaptive optics in a light sheet microscope. Optics Express, 20(12), 13252-13261. https://doi.org/10.1364/oe.20.013252
Journal Article Type | Article |
---|---|
Publication Date | Jun 1, 2012 |
Deposit Date | May 22, 2014 |
Publicly Available Date | Jul 29, 2014 |
Journal | Optics Express |
Publisher | Optica |
Peer Reviewed | Peer Reviewed |
Volume | 20 |
Issue | 12 |
Article Number | 13252-13261 |
Pages | 13252-13261 |
DOI | https://doi.org/10.1364/oe.20.013252 |
Files
Published Journal Article
(1.8 Mb)
PDF
Copyright Statement
This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OE.20.013252. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
You might also like
The Influence of In‐Channel Obstacles on River Sound
(2022)
Journal Article
Babbling brook to thunderous torrent: Using sound to monitor river stage
(2021)
Journal Article
Creating Correct Blur and its Effect on Accommodation
(2018)
Journal Article
Generating artificial reference images for open loop correlation wavefront sensors
(2018)
Journal Article
ChromaBlur: Rendering Chromatic Eye Aberration Improves Accommodation and Realism
(2017)
Journal Article
Downloadable Citations
About Durham Research Online (DRO)
Administrator e-mail: dro.admin@durham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search