5’-Deoxy-5’-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs
Skipsey, M.; Hack, G.; Hooper, T.A.; Shankey, M.C.; Conway, L.P.; Schröder, M.; Hodgson, D.R.W.
Dr Martin Schroeder email@example.com
Professor David Hodgson firstname.lastname@example.org
5′-deoxy-5′-hydrazinylguanosine was incorporated into the 5′-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5′-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.
Skipsey, M., Hack, G., Hooper, T., Shankey, M., Conway, L., Schröder, M., & Hodgson, D. (2013). 5’-Deoxy-5’-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs. Nucleosides, Nucleotides and Nucleic Acids, 32(12), 670-681. https://doi.org/10.1080/15257770.2013.851393
|Journal Article Type||Article|
|Publication Date||Dec 11, 2013|
|Deposit Date||Sep 30, 2013|
|Publicly Available Date||Jul 16, 2014|
|Journal||Nucleosides, Nucleotides and Nucleic Acids|
|Publisher||Taylor and Francis Group|
|Peer Reviewed||Peer Reviewed|
|Keywords||T7 RNA polymerase initiator, Fluorophore-labeled RNA.|
Published Journal Article
Publisher Licence URL
© Skipsey et al. Published with License by Taylor & Francis<br /> <br /> This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.<br /> <br /> Permission is granted subject to the terms of the License under which the work was published. Please check the License conditions for the work which you wish to reuse. Full and appropriate attribution must be given. This permission does not cover any third party copyrighted material which may appear in the work requested.
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