5’-Deoxy-5’-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs

Skipsey, M.; Hack, G.; Hooper, T.A.; Shankey, M.C.; Conway, L.P.; Schröder, M.; Hodgson, D.R.W.

Abstract

5′-deoxy-5′-hydrazinylguanosine was incorporated into the 5′-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5′-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.

Citation

Skipsey, M., Hack, G., Hooper, T., Shankey, M., Conway, L., Schröder, M., & Hodgson, D. (2013). 5’-Deoxy-5’-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs. Nucleosides, Nucleotides and Nucleic Acids, 32(12), 670-681. https://doi.org/10.1080/15257770.2013.851393

• Journal Article Type: Article
• Publication Date: Dec 11, 2013
• Deposit Date: Sep 30, 2013
• Publicly Available Date: Jul 16, 2014
• Journal: Nucleosides, Nucleotides and Nucleic Acids
• Print ISSN: 1525-7770
• Electronic ISSN: 1532-2335
• Publisher: Taylor and Francis Group
• Peer Reviewed: Peer Reviewed
• Volume: 32
• Issue: 12
• Pages: 670-681
• DOI: https://doi.org/10.1080/15257770.2013.851393
• Keywords: T7 RNA polymerase initiator, Fluorophore-labeled RNA.