Effect of insecticidal fusion proteins containing spider toxins targeting sodium and calcium ion channels on pyrethroid-resistant strains of peach-potato aphid (Myzus persicae)

Yang, S.; Fitches, E.; Pyati, P.; Gatehouse, J.A.

Abstract

BACKGROUND: The recombinant fusion proteins Pl1a/GNA and Hv1a/GNA contain the spider venom peptides δ-amaurobitoxin-PI1a or ω-hexatoxin-Hv1a respectively, linked to snowdrop lectin (GNA). Pl1a targets receptor site 4 of insect voltage-gated sodium channels (NaCh), while Hv1a targets voltage-gated calcium channels. Insecticide-resistant strains of peach-potato aphid (Myzus persicae) contain mutations in NaCh. The pyrethroid-resistant kdr (794J) and super-kdr (UKO) strains contain mutations at residues L1014 and M918 in the channel α-subunit respectively, while the kdr + super-kdr strain (4824J), insensitive to pyrethroids, contains mutations at both L1014 and M918. RESULTS: Pl1a/GNA and Hv1a/GNA fusion proteins have estimated LC50 values of 0.35 and 0.19 mg mL−1 when fed to wild-type M. persicae. For insecticide-resistant aphids, LC50 for the Pl1a/GNA fusion protein increased by 2–6-fold, correlating with pyrethroid resistance (wild type < kdr < super-kdr < kdr + super-kdr strains). In contrast, LC50 for the Hv1a/GNA fusion protein showed limited correlation with pyrethroid resistance. CONCLUSION: Mutations in the sodium channel in pyrethroid-resistant aphids also protect against a fusion protein containing a sodium-channel-specific toxin, in spite of differences in ligand–channel interactions, but do not confer resistance to a fusion protein targeting calcium channels. The use of fusion proteins with differing targets could play a role in managing pesticide resistance.

Citation

Yang, S., Fitches, E., Pyati, P., & Gatehouse, J. (2015). Effect of insecticidal fusion proteins containing spider toxins targeting sodium and calcium ion channels on pyrethroid-resistant strains of peach-potato aphid (Myzus persicae). Pest Management Science, 71(7), 951-956. https://doi.org/10.1002/ps.3872

• Journal Article Type: Article
• Acceptance Date: Jul 25, 2014
• Online Publication Date: Jul 31, 2014
• Publication Date: Jul 1, 2015
• Deposit Date: Feb 19, 2015
• Publicly Available Date: May 8, 2015
• Journal: Pest Management Science
• Print ISSN: 1526-498X
• Electronic ISSN: 1526-4998
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 71
• Issue: 7
• Pages: 951-956
• DOI: https://doi.org/10.1002/ps.3872
• Keywords: Biopesticide, Insecticide resistance, Homoptera/Hemiptera, Voltage-gated ion channels, Fitness cost.
• Public URL: https://durham-repository.worktribe.com/output/1445342