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Phosphate analogues in the dissection of mechanism

Korhonen, Heidi J.; Conway, Louis P.; Hodgson, David R.W.

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Authors

Heidi J. Korhonen

Louis P. Conway



Abstract

Phosphoryl group transfer is central to genetic replication, cellular signalling and many metabolic processes. Understanding the mechanisms of phosphorylation and phosphate ester and anhydride cleavage is key to efforts towards biotechnological and biomedical exploitation of phosphate-handling enzymes. Analogues of phosphate esters and anhydrides are indispensable tools, alongside protein mutagenesis and computational methods, for the dissection of phosphoryl transfer mechanisms. Hydrolysable and non-hydrolysable phosphate analogues have provided insight into the nature and sites of phosphoryl transfer processes. Kinetic isotope effects and crystallography using transition state analogues have painted more detailed pictures of transition states and how enzymes work to stabilise them.

Citation

Korhonen, H. J., Conway, L. P., & Hodgson, D. R. (2014). Phosphate analogues in the dissection of mechanism. Current Opinion in Chemical Biology, 21, 63-72. https://doi.org/10.1016/j.cbpa.2014.05.001

Journal Article Type Article
Publication Date Aug 1, 2014
Deposit Date Mar 5, 2014
Publicly Available Date Jun 16, 2014
Journal Current Opinion in Chemical Biology
Print ISSN 1367-5931
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 21
Pages 63-72
DOI https://doi.org/10.1016/j.cbpa.2014.05.001

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