Phosphate analogues in the dissection of mechanism

Korhonen, Heidi J.; Conway, Louis P.; Hodgson, David R.W.

Abstract

Phosphoryl group transfer is central to genetic replication, cellular signalling and many metabolic processes. Understanding the mechanisms of phosphorylation and phosphate ester and anhydride cleavage is key to efforts towards biotechnological and biomedical exploitation of phosphate-handling enzymes. Analogues of phosphate esters and anhydrides are indispensable tools, alongside protein mutagenesis and computational methods, for the dissection of phosphoryl transfer mechanisms. Hydrolysable and non-hydrolysable phosphate analogues have provided insight into the nature and sites of phosphoryl transfer processes. Kinetic isotope effects and crystallography using transition state analogues have painted more detailed pictures of transition states and how enzymes work to stabilise them.

Citation

Korhonen, H. J., Conway, L. P., & Hodgson, D. R. (2014). Phosphate analogues in the dissection of mechanism. Current Opinion in Chemical Biology, 21, 63-72. https://doi.org/10.1016/j.cbpa.2014.05.001

• Journal Article Type: Article
• Publication Date: Aug 1, 2014
• Deposit Date: Mar 5, 2014
• Publicly Available Date: Jun 16, 2014
• Journal: Current Opinion in Chemical Biology
• Print ISSN: 1367-5931
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 21
• Pages: 63-72
• DOI: https://doi.org/10.1016/j.cbpa.2014.05.001
• Public URL: https://durham-repository.worktribe.com/output/1437554