Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes

Carr, Rachel; Puckrin, Robert; McMahon, Brian K.; Pal, Robert; Parker, David; Pålsson, Lars-Olof

Abstract

A circularly polarized luminescence (CPL) spectrometer has been built and used to study the binding interaction of lactate and four different proteins with racemic EuIII and TbIII complexes in aqueous solution. Lactate binding gives rise to strong induced CPL spectra, and the observed emission dissymmetry factors vary linearly with enantiomeric composition. Particularly strong induced TbIII CPL also characterizes the binding interaction of alpha-1-acid glycoprotein with a dissociation constant, Kd, of 2.5 μM.

Citation

Carr, R., Puckrin, R., McMahon, B. K., Pal, R., Parker, D., & Pålsson, L. (2014). Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes. Methods and Applications in Fluorescence, 2(2), Article 024007. https://doi.org/10.1088/2050-6120/2/2/024007

• Journal Article Type: Article
• Acceptance Date: Dec 18, 2013
• Online Publication Date: Apr 9, 2014
• Publication Date: Jun 1, 2014
• Deposit Date: Jun 19, 2014
• Publicly Available Date: Dec 9, 2014
• Journal: Methods and Applications in Fluorescence
• Publisher: IOP Publishing
• Peer Reviewed: Peer Reviewed
• Volume: 2
• Issue: 2
• Article Number: 024007
• DOI: https://doi.org/10.1088/2050-6120/2/2/024007
• Public URL: https://durham-repository.worktribe.com/output/1427953