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The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells

Liu, Hebin; Schneider, Helga; Recino, Asha; Richardson, Christine; Goldberg, Martin W.; Rudd, Christopher E.

The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells Thumbnail


Authors

Hebin Liu

Helga Schneider

Asha Recino

Christine Richardson

Christopher E. Rudd



Abstract

While immune cell adaptors regulate proximal T cell signaling, direct regulation of the nuclear pore complex (NPC) has not been reported. NPC has cytoplasmic filaments composed of RanGAP1 and RanBP2 with the potential to interact with cytoplasmic mediators. Here, we show that the immune cell adaptor SLP-76 binds directly to SUMO-RanGAP1 of cytoplasmic fibrils of the NPC, and that this interaction is needed for optimal NFATc1 and NF-κB p65 nuclear entry in T cells. Transmission electron microscopy showed anti-SLP-76 cytoplasmic labeling of the majority of NPCs in anti-CD3 activated T cells. Further, SUMO-RanGAP1 bound to the N-terminal lysine 56 of SLP-76 where the interaction was needed for optimal RanGAP1-NPC localization and GAP exchange activity. While the SLP-76-RanGAP1 (K56E) mutant had no effect on proximal signaling, it impaired NF-ATc1 and p65/RelA nuclear entry and in vivo responses to OVA peptide. Overall, we have identified SLP-76 as a direct regulator of nuclear pore function in T cells.

Citation

Liu, H., Schneider, H., Recino, A., Richardson, C., Goldberg, M. W., & Rudd, C. E. (2015). The Immune Adaptor SLP-76 Binds to SUMO-RANGAP1 at Nuclear Pore Complex Filaments to Regulate Nuclear Import of Transcription Factors in T Cells. Molecular Cell, 59(5), 840-849. https://doi.org/10.1016/j.molcel.2015.07.015

Journal Article Type Article
Acceptance Date Jul 17, 2015
Online Publication Date Aug 27, 2015
Publication Date Sep 3, 2015
Deposit Date Sep 4, 2015
Publicly Available Date Oct 30, 2015
Journal Molecular Cell
Print ISSN 1097-2765
Publisher Cell Press
Peer Reviewed Peer Reviewed
Volume 59
Issue 5
Pages 840-849
DOI https://doi.org/10.1016/j.molcel.2015.07.015

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