EuroTracker® dyes : design, synthesis, structure and photophysical properties of very bright europium complexes and their use in bioassays and cellular optical imaging

Butler, S.J.; Delbianco, M.; Lamarque, L.; McMahon, B.K.; Neil, E.R.; Pal, R.; Parker, D.; Walton, J.W.; Zwier, J.

Abstract

The development of the brightest luminescent europium(III) complexes is traced, including analysis of the C3-symmetric core complex based on a functionalized triazacyclononane and identification of the most suitable strongly absorbing chromophore. Strategies for the synthesis of the complexes, including enantiopure analogues, are outlined and opportunities for applications in time-resolved microscopy and spectral imaging emphasised. Practicable examples are introduced, including selective organelle staining for cellular optical imaging at 65 nm resolution and the development of new bioassays using time-resolved FRET methods.

Citation

Butler, S., Delbianco, M., Lamarque, L., McMahon, B., Neil, E., Pal, R., …Zwier, J. (2015). EuroTracker® dyes : design, synthesis, structure and photophysical properties of very bright europium complexes and their use in bioassays and cellular optical imaging. Dalton Transactions, 44(11), 4791-4803. https://doi.org/10.1039/c4dt02785j

• Journal Article Type: Article
• Acceptance Date: Oct 10, 2014
• Online Publication Date: Oct 13, 2014
• Publication Date: Mar 21, 2015
• Deposit Date: Nov 14, 2014
• Publicly Available Date: Nov 20, 2014
• Journal: Dalton Transactions
• Print ISSN: 1477-9226
• Electronic ISSN: 1477-9234
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 44
• Issue: 11
• Pages: 4791-4803
• DOI: https://doi.org/10.1039/c4dt02785j
• Public URL: https://durham-repository.worktribe.com/output/1417672