Y. Chen
Identification of histamine receptor subtypes in skeletal myogenesis
Chen, Y.; Stegaev, V.; Kouri, V.P.; Sillat, T.; Chazot, P.L.; Stark, H.; Wen, J.G.; Konttinen, Y.T.
Authors
V. Stegaev
V.P. Kouri
T. Sillat
Professor Paul Chazot paul.chazot@durham.ac.uk
Professor
H. Stark
J.G. Wen
Y.T. Konttinen
Abstract
investigated in mouse skeletal myogenesis. Therefore, the present study aimed to investigate the HR‑subtypes in skeletal myogenesis. The myogenesis of C2C12 skeletal myoblasts was evaluated using desmin, myogenin and myosin heavy chain (Myh) as early, intermediate and late differentiation markers, respectively. Reverse transcription‑quantitative polymerase chain reaction and immunostaining were performed and the messenger RNA (mRNA) expression levels of the HR‑subtypes and markers were determined. H1R mRNA was found to be highly expressed in myoblasts at day 0; however, the expression levels were reduced as differentiation progressed. By contrast, H2R mRNA expression remained constant, while H3R mRNA expression increased by 28‑, 103‑ and 198‑fold at days 2, 4 and 6 compared with the baseline level (day 0), respectively. In addition, Myh expression increased by 7,718‑, 94,487‑ and 286,288‑fold on days 2, 4 and 6 compared with the baseline expression level (day 0). Weak positive staining of the cells for H3R protein was observed on day 2, whereas highly positive staining was observed on days 4 and 6. HR expression during myogenesis was, in part, regulated by the stage of differentiation. These results along with previous findings indicated possible involvement of H1R in the regulation of progenitor cell mitogenesis and of H2R in the relaxation of acetylcholine‑stimulated contraction of muscle cells, following the activation of professional histamine‑producing cells, including mast cells. By contrast, H3R may participate in the regulation of specialized myocyte functions, potentially by maintaining the relaxed state under the influence of constitutive H3R activity and low histamine concentrations, locally produced/released by non‑professional histamine‑producing cells.
Citation
Chen, Y., Stegaev, V., Kouri, V., Sillat, T., Chazot, P., Stark, H., …Konttinen, Y. (2015). Identification of histamine receptor subtypes in skeletal myogenesis. Molecular Medicine Reports, 11(4), 2624-2630. https://doi.org/10.3892/mmr.2014.3073
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 20, 2014 |
Online Publication Date | Dec 10, 2014 |
Publication Date | Apr 1, 2015 |
Deposit Date | May 4, 2016 |
Publicly Available Date | May 24, 2016 |
Journal | Molecular Medicine Reports |
Print ISSN | 1791-2997 |
Electronic ISSN | 1791-3004 |
Publisher | Spandidos Publications |
Peer Reviewed | Peer Reviewed |
Volume | 11 |
Issue | 4 |
Pages | 2624-2630 |
DOI | https://doi.org/10.3892/mmr.2014.3073 |
Public URL | https://durham-repository.worktribe.com/output/1413211 |
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Publisher Licence URL
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Copyright Statement
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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