Deuterated Carbohydrate Probes as ‘Label-Free’ Substrates for Probing Nutrient Uptake in Mycobacteria by Nuclear Reaction Analysis

Lowery, R.; Gibson, M.I.; Thompson, R.L.; Fullam, E.

Abstract

Understanding and probing small molecule uptake in cells is challenging, requiring sterically large chemical labels, or radioactive isotopes. Here, the uptake of deuterated sugars by Mycobacterium smegmatis, a non-pathogenic model of Mycobacterium tuberculosis, has been investigated using ion-beam (nuclear reaction) analysis demonstrating a new technique for label-free nutrient acquisition measurement.

Citation

Lowery, R., Gibson, M., Thompson, R., & Fullam, E. (2015). Deuterated Carbohydrate Probes as ‘Label-Free’ Substrates for Probing Nutrient Uptake in Mycobacteria by Nuclear Reaction Analysis. Chemical Communications, 51(23), 4838-4841. https://doi.org/10.1039/c4cc09588j

• Journal Article Type: Article
• Acceptance Date: Feb 12, 2015
• Publication Date: Mar 21, 2015
• Deposit Date: Feb 17, 2015
• Publicly Available Date: Feb 24, 2015
• Journal: Chemical Communications
• Print ISSN: 1359-7345
• Electronic ISSN: 1364-548X
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 51
• Issue: 23
• Pages: 4838-4841
• DOI: https://doi.org/10.1039/c4cc09588j
• Public URL: https://durham-repository.worktribe.com/output/1412553