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EXTRA SPINDLE POLES (Separase) controls anisotropic cell expansion in Norway spruce (Picea abies) embryos independently of its role in anaphase progression

Moschou, P.N.; Savenkov, E.I.; Minina, E.A.; Fukada, K.; Reza, S.H.; Gutierrez-Beltran, E.; Sanchez-Vera, V.; Suarez, M.F.; Hussey, P.J.; Smertenko, A.P.; Bozhkov, P.V.

EXTRA SPINDLE POLES (Separase) controls anisotropic cell expansion in Norway spruce (Picea abies) embryos independently of its role in anaphase progression Thumbnail


Authors

P.N. Moschou

E.I. Savenkov

E.A. Minina

K. Fukada

S.H. Reza

E. Gutierrez-Beltran

V. Sanchez-Vera

M.F. Suarez

A.P. Smertenko

P.V. Bozhkov



Abstract

The caspase-related protease separase (EXTRA SPINDLE POLES, ESP) plays a major role in chromatid disjunction and cell expansion in Arabidopsis thaliana. Whether the expansion phenotypes are linked to defects in cell division in Arabidopsis ESP mutants remains elusive. Here we present the identification, cloning and characterization of the gymnosperm Norway spruce (Picea abies, Pa) ESP. We used the P. abies somatic embryo system and a combination of reverse genetics and microscopy to explore the roles of Pa ESP during embryogenesis. Pa ESP was expressed in the proliferating embryonal mass, while it was absent in the suspensor cells. Pa ESP associated with kinetochore microtubules in metaphase and then with anaphase spindle midzone. During cytokinesis, it localized on the phragmoplast microtubules and on the cell plate. Pa ESP deficiency perturbed anisotropic expansion and reduced mitotic divisions in cotyledonary embryos. Furthermore, whilst Pa ESP can rescue the chromatid nondisjunction phenotype of Arabidopsis ESP mutants, it cannot rescue anisotropic cell expansion. Our data demonstrate that the roles of ESP in daughter chromatid separation and cell expansion are conserved between gymnosperms and angiosperms. However, the mechanisms of ESP-mediated regulation of cell expansion seem to be lineage-specific.

Citation

Moschou, P., Savenkov, E., Minina, E., Fukada, K., Reza, S., Gutierrez-Beltran, E., …Bozhkov, P. (2016). EXTRA SPINDLE POLES (Separase) controls anisotropic cell expansion in Norway spruce (Picea abies) embryos independently of its role in anaphase progression. New Phytologist, 212(1), 232-243. https://doi.org/10.1111/nph.14012

Journal Article Type Article
Acceptance Date Apr 7, 2016
Online Publication Date May 27, 2016
Publication Date Oct 1, 2016
Deposit Date Jul 22, 2016
Publicly Available Date May 27, 2017
Journal New Phytologist
Print ISSN 0028-646X
Electronic ISSN 1469-8137
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 212
Issue 1
Pages 232-243
DOI https://doi.org/10.1111/nph.14012
Public URL https://durham-repository.worktribe.com/output/1377647

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Copyright Statement
This is the accepted version of the following article: Moschou, P. N., Savenkov, E. I., Minina, E. A., Fukada, K., Reza, S. H., Gutierrez-Beltran, E., Sanchez-Vera, V., Suarez, M. F., Hussey, P. J., Smertenko, A. P. and Bozhkov, P. V. (2016), EXTRA SPINDLE POLES (Separase) controls anisotropic cell expansion in Norway spruce (Picea abies) embryos independently of its role in anaphase progression. New Phytologist, 212(1): 232-243, which has been published in final form at http://dx.doi.org/10.1111/nph.14012. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.






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