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Epidermal expression of a sterol biosynthesis gene regulates root growth by a non-cell autonomous mechanism in Arabidopsis

Short, E.; Pullen, M.; Imriz, G.; Liu, D.; Cope-Selby, N.; Hetherington, F.; Smertenko, A.; Hussey, P.J.; Topping, J.F.; Lindsey, K.

Epidermal expression of a sterol biosynthesis gene regulates root growth by a non-cell autonomous mechanism in Arabidopsis Thumbnail


Authors

E. Short

M. Pullen

G. Imriz

D. Liu

N. Cope-Selby

F. Hetherington

A. Smertenko



Abstract

The epidermis is hypothesized to play a signalling role during plant development. One class of mutants showing defects in signal transduction and radial patterning are those in sterol biosynthesis. The expectation is that living cells require sterols, but it is not clear that all cell types express sterol biosynthesis genes. The HYDRA1 (HYD1) gene of Arabidopsis encodes sterol Δ8-Δ7 isomerase, and although hyd1 seedlings are defective in radial patterning across several tissues, we show that the HYD1 gene is expressed most strongly in the root epidermis. Transgenic activation of HYD1 transcription in the epidermis of hyd1 null mutants reveals a major role in root patterning and growth. HYD1 expression in the vascular tissues and root meristem, though not endodermis or pericycle, also leads to some phenotypic rescue. Phenotypic rescue is associated with rescued patterning of the PIN1 and PIN2 auxin efflux carriers. The importance of the epidermis in controlling root growth and development is proposed to be in part due to its role as a site for sterol biosynthesis, and auxin is a candidate for the non-cell autonomous signal.

Journal Article Type Article
Acceptance Date Apr 16, 2018
Online Publication Date Apr 25, 2018
Publication Date May 15, 2018
Deposit Date Oct 26, 2017
Publicly Available Date Apr 27, 2018
Journal Development.
Print ISSN 0950-1991
Electronic ISSN 1477-9129
Publisher The Company of Biologists
Peer Reviewed Peer Reviewed
Volume 145
Issue 10
Article Number dev160572
DOI https://doi.org/10.1242/dev.160572
Public URL https://durham-repository.worktribe.com/output/1372952

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
© 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.






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