Induced europium CPL for the selective signalling of phosphorylated amino-acids and O-phosphorylated hexapeptides

Neil, E.R.; Pal, R.; Fox, M.A.; Parker, D.

Abstract

Two bright, europium(III) complexes based on an achiral heptadentate triazacyclononane ligand bearing two strongly absorbing chromophores have been evaluated for the selective emission and CPL signalling of various chiral O-phosphono-anions. Binding of O-phosphono-Ser and Thr gives rise to a strong induced CPL signature and a favoured Δ complex configuration is adopted. A similarly large induced CPL signal arises when [Eu·L1]2+ binds to lysophosphatidic acid (LPA), where the strong binding (log K 5.25 (295 K)) in methanol allowed its detection over the range 5 to 40 μM. Strong and chemoselective binding to the phosphorylated amino-acid residues was also observed with a set of four structurally related hexapeptides: in one case, the sign of the gem value in the ΔJ = 1 transition allowed differentiation between the binding to O-P-Ser and O-P-Tyr residues.

Citation

Neil, E., Pal, R., Fox, M., & Parker, D. (2016). Induced europium CPL for the selective signalling of phosphorylated amino-acids and O-phosphorylated hexapeptides. Dalton Transactions, 45(20), 8355-8366. https://doi.org/10.1039/c6dt01212d

• Journal Article Type: Article
• Acceptance Date: Apr 19, 2016
• Online Publication Date: Apr 20, 2016
• Publication Date: May 28, 2016
• Deposit Date: Mar 2, 2017
• Publicly Available Date: Mar 3, 2017
• Journal: Dalton Transactions
• Print ISSN: 1477-9226
• Electronic ISSN: 1477-9234
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 45
• Issue: 20
• Pages: 8355-8366
• DOI: https://doi.org/10.1039/c6dt01212d
• Public URL: https://durham-repository.worktribe.com/output/1362326