M.A. Mcdowell
Characterisation of Shigella Spa33 and Thermotoga FliM/N reveals a new model for C-ring assembly in T3SS
Mcdowell, M.A.; Marcoux, J.; Mcvicker, G.; Johnson, S.; Fong, Y.H.; Stevens, R.; Bowman, L.A.H.; Degiacomi, M.T.; Yan, J.; Wise, A.; Friede, M.E.; Benesch, J.L.P.; Deane, J.E.; Tang, C.M.; Robinson, C.V.; Lea, S.M.
Authors
J. Marcoux
G. Mcvicker
S. Johnson
Y.H. Fong
R. Stevens
L.A.H. Bowman
Matteo Degiacomi matteo.t.degiacomi@durham.ac.uk
Part Time Teacher
J. Yan
A. Wise
M.E. Friede
J.L.P. Benesch
J.E. Deane
C.M. Tang
C.V. Robinson
S.M. Lea
Abstract
Flagellar type III secretion systems (T3SS) contain an essential cytoplasmic-ring (C-ring) largely composed of two proteins FliM and FliN, whereas an analogous substructure for the closely related non-flagellar (NF) T3SS has not been observed in situ. We show that the spa33 gene encoding the putative NF-T3SS C-ring component in Shigella flexneri is alternatively translated to produce both full-length (Spa33-FL) and a short variant (Spa33-C), with both required for secretion. They associate in a 1:2 complex (Spa33-FL/C2) that further oligomerises into elongated arrays in vitro. The structure of Spa33-C2 and identification of an unexpected intramolecular pseudodimer in Spa33-FL reveal a molecular model for their higher order assembly within NF-T3SS. Spa33-FL and Spa33-C are identified as functional counterparts of a FliM–FliN fusion and free FliN respectively. Furthermore, we show that Thermotoga maritima FliM and FliN form a 1:3 complex structurally equivalent to Spa33-FL/C2, allowing us to propose a unified model for C-ring assembly by NF-T3SS and flagellar-T3SS.
Citation
Mcdowell, M., Marcoux, J., Mcvicker, G., Johnson, S., Fong, Y., Stevens, R., …Lea, S. (2016). Characterisation of Shigella Spa33 and Thermotoga FliM/N reveals a new model for C-ring assembly in T3SS. Molecular Microbiology, 99(4), 749-766. https://doi.org/10.1111/mmi.13267
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 2, 2015 |
Online Publication Date | Dec 23, 2015 |
Publication Date | Feb 12, 2016 |
Deposit Date | Jul 26, 2017 |
Publicly Available Date | Aug 7, 2017 |
Journal | Molecular Microbiology |
Print ISSN | 0950-382X |
Electronic ISSN | 1365-2958 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
Volume | 99 |
Issue | 4 |
Pages | 749-766 |
DOI | https://doi.org/10.1111/mmi.13267 |
Public URL | https://durham-repository.worktribe.com/output/1351000 |
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Copyright Statement
© 2015 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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