Víctor García-López
Molecular machines open cell membranes
García-López, Víctor; Chen, Fang; Nilewski, Lizanne G.; Duret, Guillaume; Aliyan, Amir; Kolomeisky, Anatoly B.; Robinson, Jacob T.; Wang, Gufeng; Pal, Robert; Tour, James M.
Authors
Fang Chen
Lizanne G. Nilewski
Guillaume Duret
Amir Aliyan
Anatoly B. Kolomeisky
Jacob T. Robinson
Gufeng Wang
Professor Robert Pal robert.pal@durham.ac.uk
Professor
James M. Tour
Abstract
Beyond the more common chemical delivery strategies, several physical techniques are used to open the lipid bilayers of cellular membranes. These include using electric and magnetic fields, temperature, ultrasound or light to introduce compounds into cells, to release molecular species from cells or to selectively induce programmed cell death (apoptosis) or uncontrolled cell death (necrosis). More recently, molecular motors and switches that can change their conformation in a controlled manner in response to external stimuli have been used to produce mechanical actions on tissue for biomedical applications. Here we show that molecular machines can drill through cellular bilayers using their molecular-scale actuation, specifically nanomechanical action. Upon physical adsorption of the molecular motors onto lipid bilayers and subsequent activation of the motors using ultraviolet light, holes are drilled in the cell membranes. We designed molecular motors and complementary experimental protocols that use nanomechanical action to induce the diffusion of chemical species out of synthetic vesicles, to enhance the diffusion of traceable molecular machines into and within live cells, to induce necrosis and to introduce chemical species into live cells. We also show that, by using molecular machines that bear short peptide addends, nanomechanical action can selectively target specific cell-surface recognition sites. Beyond the in vitroapplications demonstrated here, we expect that molecular machines could also be used in vivo, especially as their design progresses to allow two-photon, near-infrared and radio-frequency activation.
Citation
García-López, V., Chen, F., Nilewski, L. G., Duret, G., Aliyan, A., Kolomeisky, A. B., …Tour, J. M. (2017). Molecular machines open cell membranes. Nature, 548(7669), 567-572. https://doi.org/10.1038/nature23657
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 5, 2017 |
Online Publication Date | Aug 31, 2017 |
Publication Date | Aug 30, 2017 |
Deposit Date | Sep 25, 2017 |
Publicly Available Date | Feb 28, 2018 |
Journal | Nature |
Print ISSN | 0028-0836 |
Electronic ISSN | 1476-4687 |
Publisher | Nature Research |
Peer Reviewed | Peer Reviewed |
Volume | 548 |
Issue | 7669 |
Pages | 567-572 |
DOI | https://doi.org/10.1038/nature23657 |
Public URL | https://durham-repository.worktribe.com/output/1348606 |
Files
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