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Evidence for a duplicated mitochondrial region in Audubon’s shearwater based on MinION sequencing

Torres, L.; Welch, A.J.; Zanchetta, C.; Chesser, R.T.; Manno, M.; Donnadieu, C.; Bretagnolle, V.; Pante, E.

Evidence for a duplicated mitochondrial region in Audubon’s shearwater based on MinION sequencing Thumbnail


Authors

L. Torres

C. Zanchetta

R.T. Chesser

M. Manno

C. Donnadieu

V. Bretagnolle

E. Pante



Abstract

Mitochondrial genetic markers have been extensively used to study the phylogenetics and phylogeography of many birds, including seabirds of the order Procellariiformes. Evidence suggests that part of the mitochondrial genome of Procellariiformes, especially albatrosses, is duplicated, but no DNA fragment covering the entire duplication has been sequenced. We sequenced the complete mitochondrial genome of a non-albatross species of Procellariiformes, Puffinus lherminieri (Audubon’s shearwater) using the long-read MinION (ONT) technology. Two mitogenomes were assembled from the same individual, differing by 52 SNPs and in length. The shorter was 19 kb long while the longer was 21 kb, due to the presence of two identical copies of nad6, three tRNA, and two dissimilar copies of the control region (CR). Contrary to albatrosses, cob was not duplicated. We further detected a complex repeated region of undetermined length between the CR and 12S. Long-read sequencing suggests heteroplasmy and a novel arrangement within the duplicated region, indicating a complex evolution of the mitogenome in Procellariiformes.

Citation

Torres, L., Welch, A., Zanchetta, C., Chesser, R., Manno, M., Donnadieu, C., …Pante, E. (2019). Evidence for a duplicated mitochondrial region in Audubon’s shearwater based on MinION sequencing. Mitochondrial DNA Part A, 30(2), 256-263. https://doi.org/10.1080/24701394.2018.1484116

Journal Article Type Article
Acceptance Date May 29, 2018
Online Publication Date Jul 25, 2018
Publication Date 2019
Deposit Date May 31, 2018
Publicly Available Date Jul 25, 2019
Journal Mitochondrial DNA Part A
Print ISSN 2470-1394
Electronic ISSN 2470-1408
Publisher Taylor and Francis Group
Peer Reviewed Peer Reviewed
Volume 30
Issue 2
Pages 256-263
DOI https://doi.org/10.1080/24701394.2018.1484116
Public URL https://durham-repository.worktribe.com/output/1329728

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