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Identifying differentially expressed proteins in sorghum cell cultures exposed to osmotic stress

Ngara, Rudo; Ramulifho, Elelwani; Movahedi, Mahsa; Shargie, Nemera G.; Brown, Adrian P.; Chivasa, Stephen

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Authors

Rudo Ngara

Elelwani Ramulifho

Mahsa Movahedi

Nemera G. Shargie

Adrian P. Brown



Abstract

Drought stress triggers remarkable physiological changes and growth impediments, which significantly diminish plant biomass and crop yield. However, certain plant species show notable resilience, maintaining nearly normal yields under severe water deficits. For example, sorghum is a naturally drought-tolerant crop, which is ideal for studying plant adaptive responses to drought. Here we used sorbitol treatments to simulate drought-induced osmotic stress in sorghum cell suspension cultures and analysed fractions enriched for extracellular matrix proteins using isobaric tags for relative and absolute quantification technology. Sorbitol induced an overall increase in protein secretion, with putative redox proteins, proteases, and glycosyl hydrolases featuring prominently among the responsive proteins. Gene expression analysis of selected candidates revealed regulation at the transcriptional level. There was a notable differential gene expression between drought-tolerant and drought-sensitive sorghum varieties for some of the candidates. This study shows that protein secretion is a major component of the sorghum response to osmotic stress. Additionally, our data provide candidate genes, which may have putative functions in sorghum drought tolerance, and offer a pool of genes that could be developed as potential biomarkers for rapid identification of drought tolerant lines in plant breeding programs.

Citation

Ngara, R., Ramulifho, E., Movahedi, M., Shargie, N. G., Brown, A. P., & Chivasa, S. (2018). Identifying differentially expressed proteins in sorghum cell cultures exposed to osmotic stress. Scientific Reports, 8(1), Article 8671. https://doi.org/10.1038/s41598-018-27003-1

Journal Article Type Article
Acceptance Date May 15, 2018
Online Publication Date Jun 6, 2018
Publication Date Jun 6, 2018
Deposit Date Jun 4, 2018
Publicly Available Date Jun 4, 2018
Journal Scientific Reports
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 8
Issue 1
Article Number 8671
DOI https://doi.org/10.1038/s41598-018-27003-1

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copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
© The Author(s) 2018





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