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Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery

Wang, Pengwei; Pleskot, Roman; Zang, Jingze; Winkler, Joanna; Wang, Jie; Yperman, Klaas; Zhang, Tong; Wang, Kun; Gong, Jinli; Guan, Yajie; Richardson, Christine; Duckney, Patrick; Vandorpe, Michael; Mylle, Evelien; Fiserova, Jindriska; Van Damme, Daniel; Hussey, Patrick J.

Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery Thumbnail


Authors

Pengwei Wang

Roman Pleskot

Jingze Zang

Joanna Winkler

Jie Wang

Klaas Yperman

Tong Zhang

Kun Wang

Jinli Gong

Yajie Guan

Christine Richardson

Michael Vandorpe

Evelien Mylle

Jindriska Fiserova

Daniel Van Damme



Abstract

The Arabidopsis EH proteins (AtEH1/Pan1 and AtEH2/Pan1) are components of the endocytic TPLATE complex (TPC) which is essential for endocytosis. Both proteins are homologues of the yeast ARP2/3 complex activator, Pan1p. Here, we show that these proteins are also involved in actin cytoskeleton regulated autophagy. Both AtEH/Pan1 proteins localise to the plasma membrane and autophagosomes. Upon induction of autophagy, AtEH/Pan1 proteins recruit TPC and AP-2 subunits, clathrin, actin and ARP2/3 proteins to autophagosomes. Increased expression of AtEH/Pan1 proteins boosts autophagosome formation, suggesting independent and redundant pathways for actin-mediated autophagy in plants. Moreover, AtEHs/Pan1-regulated autophagosomes associate with ER-PM contact sites (EPCS) where AtEH1/Pan1 interacts with VAP27-1. Knock-down expression of either AtEH1/Pan1 or VAP27-1 makes plants more susceptible to nutrient depleted conditions, indicating that the autophagy pathway is perturbed. In conclusion, we identify the existence of an autophagy-dependent pathway in plants to degrade endocytic components, starting at the EPCS through the interaction among AtEH/Pan1, actin cytoskeleton and the EPCS resident protein VAP27-1.

Citation

Wang, P., Pleskot, R., Zang, J., Winkler, J., Wang, J., Yperman, K., …Hussey, P. J. (2019). Plant AtEH/Pan1 proteins drive autophagosome formation at ER-PM contact sites with actin and endocytic machinery. Nature Communications, 10(1), Article 5132. https://doi.org/10.1038/s41467-019-12782-6

Journal Article Type Article
Acceptance Date Sep 25, 2019
Online Publication Date Nov 13, 2019
Publication Date Nov 13, 2019
Deposit Date Nov 15, 2019
Publicly Available Date Nov 15, 2019
Journal Nature Communications
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 10
Issue 1
Article Number 5132
DOI https://doi.org/10.1038/s41467-019-12782-6
Public URL https://durham-repository.worktribe.com/output/1313848

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.






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