A ‘catch-and-release’ receptor for the cholera toxin

Mahon, Clare S.; Wildsmith, Gemma C.; Haksar, Diksha; de Poel, Eyleen; Beekman, Jeffrey M.; Pieters, Roland J.; Webb, Michael E.; Turnbull, W. Bruce

Abstract

Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture.

Citation

Mahon, C. S., Wildsmith, G. C., Haksar, D., de Poel, E., Beekman, J. M., Pieters, R. J., Webb, M. E., & Turnbull, W. B. (2019). A ‘catch-and-release’ receptor for the cholera toxin. Faraday Discussions, 219, 112-127. https://doi.org/10.1039/c9fd00017h

• Journal Article Type: Article
• Acceptance Date: Mar 20, 2019
• Online Publication Date: Mar 21, 2019
• Publication Date: 2019-10
• Deposit Date: Nov 19, 2019
• Journal: Faraday Discussions
• Print ISSN: 1359-6640
• Electronic ISSN: 1364-5498
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 219
• Pages: 112-127
• DOI: https://doi.org/10.1039/c9fd00017h
• Public URL: https://durham-repository.worktribe.com/output/1313523
• Related Public URLs: http://eprints.whiterose.ac.uk/149299/