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Electrochemical and thermal detection of allergenic substance lysozyme with molecularly imprinted nanoparticles

Singla, Pankaj; Kaur, Sarbjeet; Jamieson, Oliver; Dann, Amy; Garg, Saweta; Mahon, Clare; Crapnell, Robert D.; Banks, Craig E.; Kaur, Inderpreet; Peeters, Marloes

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Authors

Pankaj Singla

Sarbjeet Kaur

Oliver Jamieson

Amy Dann

Saweta Garg

Robert D. Crapnell

Craig E. Banks

Inderpreet Kaur

Marloes Peeters



Abstract

Lysozyme (LYZ) is a small cationic protein which is widely used for medical treatment and in the food industry to act as an anti-bacterial agent; however, it can trigger allergic reactions. In this study, high-affinity molecularly imprinted nanoparticles (nanoMIPs) were synthesized for LYZ using a solid-phase approach. The produced nanoMIPs were electrografted to screen-printed electrodes (SPEs), disposable electrodes with high commercial potential, to enable electrochemical and thermal sensing. Electrochemical impedance spectroscopy (EIS) facilitated fast measurement (5–10 min) and is able to determine trace levels of LYZ (pM) and can discriminate between LYZ and structurally similar proteins (bovine serum albumin, troponin-I). In tandem, thermal analysis was conducted with the heat transfer method (HTM), which is based on monitoring the heat transfer resistance at the solid–liquid interface of the functionalized SPE. HTM as detection technique guaranteed trace-level (fM) detection of LYZ but needed longer analysis time compared to EIS measurement (30 min vs 5–10 min). Considering the versatility of the nanoMIPs which can be adapted to virtually any target of interest, these low-cost point-of-care sensors hold great potential to improve food safety.

Citation

Singla, P., Kaur, S., Jamieson, O., Dann, A., Garg, S., Mahon, C., …Peeters, M. (2023). Electrochemical and thermal detection of allergenic substance lysozyme with molecularly imprinted nanoparticles. Analytical and Bioanalytical Chemistry, 415(18), 4467-4478. https://doi.org/10.1007/s00216-023-04638-2

Journal Article Type Article
Acceptance Date Feb 25, 2023
Online Publication Date Mar 11, 2023
Publication Date 2023-07
Deposit Date Oct 16, 2023
Publicly Available Date Oct 16, 2023
Journal Analytical and Bioanalytical Chemistry
Print ISSN 1618-2642
Electronic ISSN 1618-2650
Publisher Springer
Peer Reviewed Peer Reviewed
Volume 415
Issue 18
Pages 4467-4478
DOI https://doi.org/10.1007/s00216-023-04638-2
Keywords Biochemistry; Analytical Chemistry
Public URL https://durham-repository.worktribe.com/output/1803993

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Licence
http://creativecommons.org/licenses/by/4.0/

Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.





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