Holographic projection for multi-spot structured illumination microscopy

Ward, E.N.; Pal, R.

Abstract

This paper describes the design and testing of a multi‐spot structured illumination microscopy system using computer‐generated holograms to create the required excitation patterns. Furthermore, it demonstrates the use of an adapted direct search algorithm for calculating the holograms that allows for imaging across an extended field of view. The system was tested on fixed targets and live cells yielding a two times resolution increase over conventional diffraction‐limited imaging.

Citation

Ward, E., & Pal, R. (2019). Holographic projection for multi-spot structured illumination microscopy. Journal of Microscopy, 274(2), 114-120. https://doi.org/10.1111/jmi.12787

• Journal Article Type: Article
• Acceptance Date: Feb 19, 2019
• Online Publication Date: Feb 21, 2019
• Publication Date: May 31, 2019
• Deposit Date: Feb 22, 2019
• Publicly Available Date: Feb 21, 2020
• Journal: Journal of Microscopy
• Print ISSN: 0022-2720
• Electronic ISSN: 1365-2818
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 274
• Issue: 2
• Pages: 114-120
• DOI: https://doi.org/10.1111/jmi.12787
• Public URL: https://durham-repository.worktribe.com/output/1307363