Split-QF System for Fine-Tuned Transgene Expression in Drosophila

Riabinina, O.; Vernon, S.W.; Dickson, B.J.; Baines, R.A.

Abstract

The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila, is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines, thus greatly expanding the range of possible advanced intersectional experiments and anatomical, physiological, and behavioral assays in Drosophila, and in other organisms.

Citation

Riabinina, O., Vernon, S., Dickson, B., & Baines, R. (2019). Split-QF System for Fine-Tuned Transgene Expression in Drosophila. Genetics, 212(1), 53-63. https://doi.org/10.1534/genetics.119.302034

• Journal Article Type: Article
• Acceptance Date: Mar 3, 2019
• Online Publication Date: May 3, 2019
• Publication Date: May 31, 2019
• Deposit Date: May 16, 2019
• Publicly Available Date: May 22, 2019
• Journal: Genetics
• Print ISSN: 0016-6731
• Electronic ISSN: 1943-2631
• Publisher: Genetics Society of America
• Peer Reviewed: Peer Reviewed
• Volume: 212
• Issue: 1
• Pages: 53-63
• DOI: https://doi.org/10.1534/genetics.119.302034
• Public URL: https://durham-repository.worktribe.com/output/1301305