Klaus Suhling
Wide-field time-correlated single photon counting-based fluorescence lifetime imaging microscopy
Suhling, Klaus; Hirvonen, Liisa M.; Becker, Wolfgang; Smietana, Stefan; Netz, Holger; Milnes, James; Conneely, Thomas; Marois, Alix Le; Jagutzki, Ottmar; Festy, Fred; Petrášek, Zdeněk; Beeby, Andrew
Authors
Liisa M. Hirvonen
Wolfgang Becker
Stefan Smietana
Holger Netz
James Milnes
Thomas Conneely
Alix Le Marois
Ottmar Jagutzki
Fred Festy
Zdeněk Petrášek
Professor Andrew Beeby andrew.beeby@durham.ac.uk
Professor
Abstract
Wide-field time-correlated single photon counting detection techniques, where the position and the arrival time of the photons are recorded simultaneously using a camera, have made some advances recently. The technology and instrumentation used for this approach is employed in areas such as nuclear science, mass spectroscopy and positron emission tomography, but here, we discuss some of the wide-field TCSPC methods, for applications in fluorescence microscopy. We describe work by us and others as presented in the Ulitima fast imaging and tracking conference at the Argonne National Laboratory in September 2018, from phosphorescence lifetime imaging (PLIM) microscopy on the microsecond time scale to fluorescence lifetime imaging (FLIM) on the nanosecond time scale, and highlight some applications of these techniques.
Citation
Suhling, K., Hirvonen, L. M., Becker, W., Smietana, S., Netz, H., Milnes, J., …Beeby, A. (2019). Wide-field time-correlated single photon counting-based fluorescence lifetime imaging microscopy. Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 942, Article 162365. https://doi.org/10.1016/j.nima.2019.162365
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 10, 2019 |
Online Publication Date | Jul 15, 2019 |
Publication Date | Oct 21, 2019 |
Deposit Date | Aug 29, 2019 |
Publicly Available Date | Aug 29, 2019 |
Journal | Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment |
Print ISSN | 0168-9002 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 942 |
Article Number | 162365 |
DOI | https://doi.org/10.1016/j.nima.2019.162365 |
Public URL | https://durham-repository.worktribe.com/output/1289353 |
Files
Published Journal Article
(1.9 Mb)
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
Copyright Statement
© 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license
(http://creativecommons.org/licenses/by/4.0/).
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