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Cell-intrinsic and -extrinsic mechanisms promote cell-type-specific cytokinetic diversity

Davies, Tim; Kim, Han X; Romano Spica, Natalia; Lesea-Pringle, Benjamin J; Dumont, Julien; Shirasu-Hiza, Mimi; Canman, Julie C

Cell-intrinsic and -extrinsic mechanisms promote cell-type-specific cytokinetic diversity Thumbnail


Authors

Han X Kim

Natalia Romano Spica

Benjamin J Lesea-Pringle

Julien Dumont

Mimi Shirasu-Hiza

Julie C Canman



Abstract

Cytokinesis, the physical division of one cell into two, is powered by constriction of an actomyosin contractile ring. It has long been assumed that all animal cells divide by a similar molecular mechanism, but growing evidence suggests that cytokinetic regulation in individual cell types has more variation than previously realized. In the four-cell Caenorhabditis elegans embryo, each blastomere has a distinct cell fate, specified by conserved pathways. Using fast-acting temperature-sensitive mutants and acute drug treatment, we identified cell-type-specific variation in the cytokinetic requirement for a robust forminCYK-1-dependent filamentous-actin (F-actin) cytoskeleton. In one cell (P2), this cytokinetic variation is cell-intrinsically regulated, whereas in another cell (EMS) this variation is cell-extrinsically regulated, dependent on both SrcSRC-1 signaling and direct contact with its neighbor cell, P2. Thus, both cell-intrinsic and -extrinsic mechanisms control cytokinetic variation in individual cell types and can protect against division failure when the contractile ring is weakened.

Citation

Davies, T., Kim, H. X., Romano Spica, N., Lesea-Pringle, B. J., Dumont, J., Shirasu-Hiza, M., & Canman, J. C. (2018). Cell-intrinsic and -extrinsic mechanisms promote cell-type-specific cytokinetic diversity. eLife, 7, Article e36204. https://doi.org/10.7554/elife.36204

Journal Article Type Article
Acceptance Date Jun 10, 2018
Online Publication Date Jul 20, 2018
Publication Date Jul 20, 2018
Deposit Date Jan 14, 2020
Publicly Available Date Feb 16, 2020
Journal eLife
Publisher eLife Sciences Publications
Peer Reviewed Peer Reviewed
Volume 7
Article Number e36204
DOI https://doi.org/10.7554/elife.36204
Public URL https://durham-repository.worktribe.com/output/1273760

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
© 2018, Davies et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.






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