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CYK4 Promotes Antiparallel Microtubule Bundling by Optimizing MKLP1 Neck Conformation

Davies, Tim; Kodera, Noriyuki; Kaminski Schierle, Gabriele S.; Rees, Eric; Erdelyi, Miklos; Kaminski, Clemens F.; Ando, Toshio; Mishima, Masanori

Authors

Noriyuki Kodera

Gabriele S. Kaminski Schierle

Eric Rees

Miklos Erdelyi

Clemens F. Kaminski

Toshio Ando

Masanori Mishima



Abstract

Centralspindlin, a constitutive 2:2 heterotetramer of MKLP1 (a kinesin-6) and the non-motor subunit CYK4, plays important roles in cytokinesis. It is crucial for the formation of central spindle microtubule bundle structure. Its accumulation at the central antiparallel overlap zone is key for recruitment and regulation of downstream cytokinesis factors and for stable anchoring of the plasma membrane at the midbody. Both MKLP1 and CYK4 are required for efficient microtubule bundling. However, the mechanism by which CYK4 contributes to this is unclear. Here we performed structural and functional analyses of centralspindlin using high-speed atomic force microscopy, Fӧrster resonance energy transfer analysis, and in vitro reconstitution. Our data reveal that CYK4 binds to a globular mass in the atypically long MKLP1 neck domain between the catalytic core and the coiled coil and thereby reconfigures the two motor domains in the MKLP1 dimer to be suitable for antiparallel microtubule bundling. Our work provides insights into the microtubule bundling during cytokinesis and into the working mechanisms of the kinesins with non-canonical neck structures.

Citation

Davies, T., Kodera, N., Kaminski Schierle, G. S., Rees, E., Erdelyi, M., Kaminski, C. F., …Mishima, M. (2015). CYK4 Promotes Antiparallel Microtubule Bundling by Optimizing MKLP1 Neck Conformation. PLoS Biology, 13(4), Article e1002121. https://doi.org/10.1371/journal.pbio.1002121

Journal Article Type Article
Acceptance Date Mar 5, 2015
Online Publication Date Apr 13, 2015
Publication Date 2015
Deposit Date Jan 14, 2020
Journal PLoS Biology
Print ISSN 1544-9173
Electronic ISSN 1545-7885
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 13
Issue 4
Article Number e1002121
DOI https://doi.org/10.1371/journal.pbio.1002121
Public URL https://durham-repository.worktribe.com/output/1273732