N-Terminal speciation for native chemical ligation

Maguire, Oliver R.; Zhu, Jiayun; Brittain, William D.G.; Hudson, Alexander S.; Cobb, Steven L.; O’Donoghue, AnnMarie C.

Abstract

Native chemical ligation (NCL) enables the chemical synthesis of peptides via reactions between N-terminal thiolates and C-terminal thioesters under mild, aqueous conditions at pH 7–8. Here we demonstrate quantitatively how thiol speciation at N-terminal cysteines and analogues varies significantly depending upon structure at typical pH values used in NCL.

Citation

Maguire, O. R., Zhu, J., Brittain, W. D., Hudson, A. S., Cobb, S. L., & O’Donoghue, A. C. (2020). N-Terminal speciation for native chemical ligation. Chemical Communications, 56(45), 6114-6117. https://doi.org/10.1039/d0cc01604g

• Journal Article Type: Article
• Acceptance Date: Apr 21, 2020
• Online Publication Date: Apr 23, 2020
• Publication Date: 2020-06
• Deposit Date: Jun 10, 2020
• Publicly Available Date: Jun 10, 2020
• Journal: Chemical Communications
• Print ISSN: 1359-7345
• Electronic ISSN: 1364-548X
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 56
• Issue: 45
• Pages: 6114-6117
• DOI: https://doi.org/10.1039/d0cc01604g
• Public URL: https://durham-repository.worktribe.com/output/1269016