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A DNA-binding bromodomain-containing protein interacts with and reduces Rx1-mediated immune response to Potato Virus X

Sukarta, Octavina C.A.; Townsend, Philip D; Llewelyn, Alexander; Dixon, Christopher H; Slootweg, Erik J; Pålsson, Lars-Olof; Takken, Frank L.W.; Goverse, Aska; Cann, Martin J

A DNA-binding bromodomain-containing protein interacts with and reduces Rx1-mediated immune response to Potato Virus X Thumbnail


Authors

Octavina C.A. Sukarta

Philip D Townsend

Alexander Llewelyn

Christopher H Dixon

Erik J Slootweg

Lars-Olof Pålsson

Frank L.W. Takken

Aska Goverse



Abstract

Plant NLR proteins enable the immune system to recognise and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming. Some NLRs have been shown to act in the nucleus and interact with transcription factors. The Rx1 NLR protein of potato binds and distorts double-stranded DNA. However, the components of the chromatin localized Rx1-complex are largely unknown. Here we report a physical and functional interaction between Rx1 and NbDBCP, a bromodomain-containing chromatin-interacting protein. NbDBCP accumulates in the nucleolus, interacts with chromatin and redistributes Rx1 to the nucleolus in a subpopulation of imaged cells. Rx1 over-expression reduces NbDBCP interactions with chromatin. NbDBCP is a negative regulator of Rx1-mediated immune responses to potato virus X (PVX) and this activity requires an intact bromodomain. Previously, Rx1 has been shown to regulate the DNA-binding activity of a Golden2-like transcription factor, NbGlk1. Rx1 and NbDBCP act synergistically to reduce NbGlk1 DNA-binding suggesting a mode of action for NbDBCP’s inhibitory effect on immunity. This study provides new mechanistic insight into how a chromatin localised NLR complex co-ordinates immune signalling following pathogen perception.

Citation

Sukarta, O. C., Townsend, P. D., Llewelyn, A., Dixon, C. H., Slootweg, E. J., Pålsson, L., …Cann, M. J. (2020). A DNA-binding bromodomain-containing protein interacts with and reduces Rx1-mediated immune response to Potato Virus X. Plant communications, 1(4), Article 100086. https://doi.org/10.1016/j.xplc.2020.100086

Journal Article Type Article
Acceptance Date Jun 11, 2020
Online Publication Date Jun 16, 2020
Publication Date Jun 13, 2020
Deposit Date Jun 11, 2020
Publicly Available Date Jul 14, 2020
Journal Plant communications.
Publisher Cell Press
Peer Reviewed Peer Reviewed
Volume 1
Issue 4
Article Number 100086
DOI https://doi.org/10.1016/j.xplc.2020.100086
Public URL https://durham-repository.worktribe.com/output/1268612

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