Synthesis and Evaluation of Europium Complexes that Switch On Luminescence in Lysosomes of Living Cells

Starck, Matthieu; Fradgley, Jack D.; Pal, Robert; Zwier, Jurriaan M.; Lamarque, Laurent; Parker, David

Abstract

A set of four luminescent Eu(III) complexes bearing an extended aryl‐alkynylpyridine chromophore has been studied, showing very different pH‐dependent behaviour in their absorption and emission spectral response. For two complexes with p K a values of 6.45 and 6.20 in protein containing solution, the emission lifetime increases very significantly following protonation. By varying the gate time during signal acquisition, the ‘switch‐on’ intensity ratio could be optimised and enhancement factors of between 250 to 1330 were measured between pH 8 and 4. The best behaved probe showed no dependence on the concentration of common endogenous cations, selected reductants and serum albumin and was examined in live cell imaging studies and used to monitor time‐dependent lysosomal acidification, for which the increase in observed image brightness due to acidification was found to be a factor of 50 in NIH‐3T3 cells.

Citation

Starck, M., Fradgley, J. D., Pal, R., Zwier, J. M., Lamarque, L., & Parker, D. (2021). Synthesis and Evaluation of Europium Complexes that Switch On Luminescence in Lysosomes of Living Cells. Chemistry - A European Journal, 27(2), 766-777. https://doi.org/10.1002/chem.202003992

• Journal Article Type: Article
• Acceptance Date: Nov 11, 2020
• Online Publication Date: Dec 15, 2020
• Publication Date: Jan 7, 2021
• Deposit Date: Nov 17, 2020
• Publicly Available Date: Mar 2, 2021
• Journal: Chemistry - A European Journal
• Print ISSN: 0947-6539
• Electronic ISSN: 1521-3765
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 27
• Issue: 2
• Pages: 766-777
• DOI: https://doi.org/10.1002/chem.202003992
• Public URL: https://durham-repository.worktribe.com/output/1257174