Statistical reproducibility for pairwise t-tests in pharmaceutical research

Simkus, A.; Coolen, F.P.A.; Coolen-Maturi, T.; Karp, N.A.; Bendtsen, C.

Abstract

This paper investigates statistical reproducibility of the t-test. We formulate reproducibility as a predictive inference problem and apply the nonparametric predictive inference (NPI) method. Within our research framework, statistical reproducibility provides inference on the probability that the same test outcome would be reached, if the test were repeated under identical conditions. We present an NPI algorithm to calculate the reproducibility of the t-test and then use simulations to explore the reproducibility both under the null and alternative hypotheses. We then apply NPI reproducibility to a real life scenario of a preclinical experiment, which involves multiple pairwise comparisons of test groups, where different groups are given a different concentration of a drug. The aim of the experiment is to decide the concentration of the drug which is most effective. In both simulations and the application scenario, we study the relationship between reproducibility and two test statistics, the Cohen’s d and the p-value. We also compare the reproducibility of the t-test with the reproducibility of the Wilcoxon Mann-Whitney test. Finally, we examine reproducibility for the final decision of choosing a particular dose in the multiple pairwise comparisons scenario. This paper presents advances on the topic of test reproducibility with relevance for tests used in pharmaceutical research.

Citation

Simkus, A., Coolen, F., Coolen-Maturi, T., Karp, N., & Bendtsen, C. (2022). Statistical reproducibility for pairwise t-tests in pharmaceutical research. Statistical Methods in Medical Research, 31(4), 673-688. https://doi.org/10.1177/09622802211041765

• Journal Article Type: Article
• Acceptance Date: Jul 31, 2021
• Online Publication Date: Dec 2, 2021
• Publication Date: Apr 1, 2022
• Deposit Date: Jul 31, 2021
• Publicly Available Date: Aug 2, 2021
• Journal: Statistical Methods in Medical Research
• Print ISSN: 0962-2802
• Electronic ISSN: 1477-0334
• Publisher: SAGE Publications
• Peer Reviewed: Peer Reviewed
• Volume: 31
• Issue: 4
• Pages: 673-688
• DOI: https://doi.org/10.1177/09622802211041765
• Public URL: https://durham-repository.worktribe.com/output/1238430