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Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution

Williams, Gareth O.S.; Williams, Elvira; Finlayson, Neil; Erdogan, Ahmet T.; Wang, Qiang; Fernandes, Susan; Akram, Ahsan R.; Dhaliwal, Kev; Henderson, Robert K.; Girkin, John M.; Bradley, Mark

Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution Thumbnail


Authors

Gareth O.S. Williams

Elvira Williams

Neil Finlayson

Ahmet T. Erdogan

Qiang Wang

Susan Fernandes

Ahsan R. Akram

Kev Dhaliwal

Robert K. Henderson

Mark Bradley



Abstract

The use of optical techniques to interrogate wide ranging samples from semiconductors to biological tissue for rapid analysis and diagnostics has gained wide adoption over the past decades. The desire to collect ever more spatially, spectrally and temporally detailed optical signatures for sample characterization has specifically driven a sharp rise in new optical microscopy technologies. Here we present a high-speed optical scanning microscope capable of capturing time resolved images across 512 spectral and 32 time channels in a single acquisition with the potential for ~0.2 frames per second (256 × 256 image pixels). Each pixel in the resulting images contains a detailed data cube for the study of diverse time resolved light driven phenomena. This is enabled by integration of system control electronics and on-chip processing which overcomes the challenges presented by high data volume and low imaging speed, often bottlenecks in previous systems.

Citation

Williams, G. O., Williams, E., Finlayson, N., Erdogan, A. T., Wang, Q., Fernandes, S., …Bradley, M. (2021). Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution. Nature Communications, 12, Article 6616. https://doi.org/10.1038/s41467-021-26837-0

Journal Article Type Article
Acceptance Date Oct 15, 2021
Online Publication Date Nov 16, 2021
Publication Date 2021
Deposit Date Nov 18, 2021
Publicly Available Date Nov 18, 2021
Journal Nature Communications
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 12
Article Number 6616
DOI https://doi.org/10.1038/s41467-021-26837-0

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.






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