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Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution

Williams, Gareth O.S.; Williams, Elvira; Finlayson, Neil; Erdogan, Ahmet T.; Wang, Qiang; Fernandes, Susan; Akram, Ahsan R.; Dhaliwal, Kev; Henderson, Robert K.; Girkin, John M.; Bradley, Mark

Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution Thumbnail


Gareth O.S. Williams

Elvira Williams

Neil Finlayson

Ahmet T. Erdogan

Qiang Wang

Susan Fernandes

Ahsan R. Akram

Kev Dhaliwal

Robert K. Henderson

Mark Bradley


The use of optical techniques to interrogate wide ranging samples from semiconductors to biological tissue for rapid analysis and diagnostics has gained wide adoption over the past decades. The desire to collect ever more spatially, spectrally and temporally detailed optical signatures for sample characterization has specifically driven a sharp rise in new optical microscopy technologies. Here we present a high-speed optical scanning microscope capable of capturing time resolved images across 512 spectral and 32 time channels in a single acquisition with the potential for ~0.2 frames per second (256 × 256 image pixels). Each pixel in the resulting images contains a detailed data cube for the study of diverse time resolved light driven phenomena. This is enabled by integration of system control electronics and on-chip processing which overcomes the challenges presented by high data volume and low imaging speed, often bottlenecks in previous systems.


Williams, G. O., Williams, E., Finlayson, N., Erdogan, A. T., Wang, Q., Fernandes, S., …Bradley, M. (2021). Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution. Nature Communications, 12, Article 6616.

Journal Article Type Article
Acceptance Date Oct 15, 2021
Online Publication Date Nov 16, 2021
Publication Date 2021
Deposit Date Nov 18, 2021
Publicly Available Date Nov 18, 2021
Journal Nature Communications
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 12
Article Number 6616


Published Journal Article (5.5 Mb)

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