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A comprehensive structural analysis of the ATPase domain of Human DNA topoisomerase II Beta bound to AMPPNP, ADP and the bisdioxopiperazine, ICRF193

Ling, E.M.; Baslé, A.; Cowell, I.G.; Van Den Berg, B.; Blower, T.R.; Austin, C.A.

A comprehensive structural analysis of the ATPase domain of Human DNA topoisomerase II Beta bound to AMPPNP, ADP and the bisdioxopiperazine, ICRF193 Thumbnail


Authors

E.M. Ling

A. Baslé

I.G. Cowell

B. Van Den Berg

C.A. Austin



Abstract

Human topoisomerase II beta (TOP2B) modulates DNA topology using energy from ATP hydrolysis. To investigate the conformational changes that occur during ATP hydrolysis, we determined the X-ray crystallographic structures of the human TOP2B ATPase domain bound to AMPPNP or ADP at 1.9 Å and 2.6 Å resolution, respectively. The GHKL domains of both structures are similar, whereas the QTK loop within the transducer domain can move for product release. As TOP2B is the clinical target of bisdioxopiperazines, we also determined the structure of a TOP2B:ADP:ICRF193 complex to 2.3 Å resolution and identified key drug-binding residues. Biochemical characterization revealed the N-terminal strap reduces the rate of ATP hydrolysis. Mutagenesis demonstrated residue E103 as essential for ATP hydrolysis in TOP2B. Our data provide fundamental insights into the tertiary structure of the human TOP2B ATPase domain and a potential regulatory mechanism for ATP hydrolysis.

Citation

Ling, E., Baslé, A., Cowell, I., Van Den Berg, B., Blower, T., & Austin, C. (2022). A comprehensive structural analysis of the ATPase domain of Human DNA topoisomerase II Beta bound to AMPPNP, ADP and the bisdioxopiperazine, ICRF193. Structure, 30(8), P1129-1145.e3. https://doi.org/10.1016/j.str.2022.05.009

Journal Article Type Article
Acceptance Date May 10, 2022
Online Publication Date Jun 3, 2022
Publication Date Aug 4, 2022
Deposit Date May 5, 2022
Publicly Available Date Jan 31, 2023
Journal Structure
Print ISSN 0969-2126
Electronic ISSN 1878-4186
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 30
Issue 8
Pages P1129-1145.e3
DOI https://doi.org/10.1016/j.str.2022.05.009
Public URL https://durham-repository.worktribe.com/output/1208763

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