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Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism

Liao, Pan; Lechon, Tamara; Romsdahl, Trevor; Woodfield, Helen; Fenyk, Stepan; Fawcett, Tony; Wallington, Emma; Bates, Ruth E.; Chye, Mee-Len; Chapman, Kent D.; Harwood, John L.; Scofield, Simon

Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism Thumbnail


Authors

Pan Liao

Tamara Lechon

Trevor Romsdahl

Helen Woodfield

Stepan Fenyk

Emma Wallington

Ruth E. Bates

Mee-Len Chye

Kent D. Chapman

John L. Harwood

Simon Scofield



Abstract

Oilseed rape (Brassica napus) is an important crop that is cultivated for the oil (mainly triacylglycerol; TAG) it produces in its seeds. TAG synthesis is controlled mainly by key enzymes in the Kennedy pathway, such as glycerol 3-phosphate acyltransferase (GPAT), lysophosphatidate acyltransferase (LPAT) and diacylglycerol acyltransferase (DGAT) but can also be produced from phosphoglycerides such as phosphatidylcholine (PC) by the activity of the enzyme phospholipid: diacylglycerol acyltransferase (PDAT). To evaluate the potential for these enzymes to alter oil yields or composition, we analysed transgenic B. napus lines which overexpressed GPAT, LPAT or PDAT using heterologous transgenes from Arabidopsis and Nasturtium and examined lipid profiles and changes in gene expression in these lines compared to WT. Distinct changes in PC and TAG abundance and spatial distribution in embryonic tissues were observed in some of the transgenic lines, together with altered expression of genes involved generally in acyl-lipid metabolism. Overall our results show that up-regulation of these key enzymes differentially affects lipid composition and distribution as well as lipid-associated gene expression, providing important information which could be used to improve crop properties by metabolic engineering.

Citation

Liao, P., Lechon, T., Romsdahl, T., Woodfield, H., Fenyk, S., Fawcett, T., Wallington, E., Bates, R. E., Chye, M.-L., Chapman, K. D., Harwood, J. L., & Scofield, S. (2022). Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism. Scientific Reports, 12(1), https://doi.org/10.1038/s41598-022-07387-x

Journal Article Type Article
Acceptance Date Feb 17, 2022
Online Publication Date Mar 1, 2022
Publication Date 2022
Deposit Date May 17, 2022
Publicly Available Date May 17, 2022
Journal Scientific Reports
Publisher Nature Research
Peer Reviewed Peer Reviewed
Volume 12
Issue 1
DOI https://doi.org/10.1038/s41598-022-07387-x
Public URL https://durham-repository.worktribe.com/output/1207273

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.





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