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Extracellular ATP targets Arabidopsis RIBONUCLEASE 1 to suppress mycotoxin stress-induced cell death

Goodman, Heather L.; Kroon, Johan T.M.; Tomé, Daniel F.A.; Hamilton, John M.U.; Alqarni, Ali O.; Chivasa, Stephen

Extracellular ATP targets Arabidopsis RIBONUCLEASE 1 to suppress mycotoxin stress-induced cell death Thumbnail


Authors

Heather L. Goodman

Daniel F.A. Tomé

John M.U. Hamilton

Ali Alqarni ali.alqarni@durham.ac.uk
PGR Student Doctor of Philosophy



Abstract

Extracellular ATP is a purinergic signal with important functions in regulating plant growth and stress-adaptive responses, including programmed cell death. While signalling events proximate to receptor activation at the plasma membrane have been characterised, downstream protein targets and the mechanism of cell death activation/regulation are unknown. We designed a proteomic screen to identify ATP-responsive proteins in Arabidopsis cell cultures exposed to mycotoxin stress via fumonisin B1 (FB1) application. Arabidopsis RIBONUCLEASE 1 (RNS1) was identified by the screen and transgenic plants overexpressing native RNS1showed greater susceptibility to FB1, while a gene knockout rns1 mutant and antisense RNS1 transgenic plants were resistant to FB1-induced cell death. Native RNS1 complemented rns1 mutants and restored cell death response to FB1, while a catalytically inactive version of the ribonuclease could not. The FB1 resistance of salicylic acid-depleted nahG-expressing plants was abolished by transformation with native RNS1, but not the catalytically dead version. The mechanism of FB1-induced cell death is activation of RNS1-dependent RNA cleavage, which is blocked by ATP via RNS1 suppression, or enhanced by salicylic acid (SA) through induction of RNS1 expression. Our study reveals RNS1 as a previously unknown convergence point of ATP and SA signalling in the regulation of stress-induced cell death.

Journal Article Type Article
Acceptance Date Apr 29, 2022
Online Publication Date May 31, 2022
Publication Date 2022-08
Deposit Date May 6, 2022
Publicly Available Date Jul 25, 2022
Journal New Phytologist
Print ISSN 0028-646X
Electronic ISSN 1469-8137
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 235
Issue 4
Pages 1531-1542
DOI https://doi.org/10.1111/nph.18211
Public URL https://durham-repository.worktribe.com/output/1206816

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.





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