Mohit Sharma
Shape-Morphing of an Artificial Protein Cage with Unusual Geometry Induced by a Single Amino Acid Change
Sharma, Mohit; Biela, Artur P.; Kowalczyk, Agnieszka; Borzęcka-Solarz, Kinga; Piette, Bernard M.A.G.; Gaweł, Szymon; Bishop, Joshua; Kukura, Philipp; Benesch, Justin L.P.; Imamura, Motonori; Scheuring, Simon; Heddle, Jonathan G.
Authors
Artur P. Biela
Agnieszka Kowalczyk
Kinga Borzęcka-Solarz
Professor Bernard Piette b.m.a.g.piette@durham.ac.uk
Professor
Szymon Gaweł
Joshua Bishop
Philipp Kukura
Justin L.P. Benesch
Motonori Imamura
Simon Scheuring
Jonathan G. Heddle
Abstract
Artificial protein cages are constructed from multiple protein subunits. The interaction between the subunits, notably the angle formed between them, controls the geometry of the resulting cage. Here, using the artificial protein cage, “TRAP-cage”, we show that a simple alteration in the position of a single amino acid responsible for Au(I)-mediated subunit–subunit interactions in the constituent ring-shaped building blocks results in a more acute dihedral angle between them. In turn, this causes a dramatic shift in the structure from a 24-ring cage with an octahedral symmetry to a 20-ring cage with a C2 symmetry. This symmetry change is accompanied by a decrease in the number of Au(I)-mediated bonds between cysteines and a concomitant change in biophysical properties of the cage.
Citation
Sharma, M., Biela, A. P., Kowalczyk, A., Borzęcka-Solarz, K., Piette, B. M., Gaweł, S., …Heddle, J. G. (2022). Shape-Morphing of an Artificial Protein Cage with Unusual Geometry Induced by a Single Amino Acid Change. ACS Nanoscience Au, 2(5), 404-413. https://doi.org/10.1021/acsnanoscienceau.2c00019
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 22, 2022 |
Online Publication Date | May 9, 2022 |
Publication Date | Oct 19, 2022 |
Deposit Date | May 27, 2022 |
Publicly Available Date | Dec 15, 2022 |
Journal | ACS Nanoscience Au |
Electronic ISSN | 2694-2496 |
Publisher | American Chemical Society |
Peer Reviewed | Peer Reviewed |
Volume | 2 |
Issue | 5 |
Pages | 404-413 |
DOI | https://doi.org/10.1021/acsnanoscienceau.2c00019 |
Public URL | https://durham-repository.worktribe.com/output/1202857 |
Files
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
Copyright Statement
© 2022 The Authors. Published by American Chemical Society.
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