Preparation and thermally-induced self-assembly behaviour of elastin-like peptide side-chain polymer-gold nanoparticle (ESP-GNP) hybrids

Zong, Jingyi; Cobb, Steven L.; Cameron, Neil R.

Abstract

Thermoresponsive gold nanoparticles were prepared by conjugation of short pentapeptide sequences, containing the elastin-like peptide (ELP) repeat unit Val-Pro-Gly-Val-Gly (VPGVG), to a well-defined active ester prepolymer, poly(pentafluorophenyl acrylate) (pPFPA), itself prepared by RAFT polymerisation. Polymers with number-average chain lengths of 25, 50, 75 and 100 were prepared. The cloud points of their aqueous solutions was found to decrease with chain length and peptide sequence, and varied from 76 to 42 oC. Circular dichroism indicated that the cloud point behaviour was accompanied by a reversible change in peptide conformation from a random coil to a -turn. The resulting thermoresponsive elastin peptide side-chain polymers (ESPs) were then conjugated to gold nanoparticles (GNPs) by ligand exchange. The resulting ESPGNPs similarly displayed thermo-reversible aggregation with transition temperature (Tt) values of 65 and 35 oC for ESPs of chain length 75 and 100, respectively.

Citation

Zong, J., Cobb, S. L., & Cameron, N. R. (2021). Preparation and thermally-induced self-assembly behaviour of elastin-like peptide side-chain polymer-gold nanoparticle (ESP-GNP) hybrids. ARKIVOC, 2021(6), 131 - 147. https://doi.org/10.24820/ark.5550190.p011.589

• Journal Article Type: Article
• Acceptance Date: Aug 7, 2021
• Online Publication Date: Aug 16, 2021
• Publication Date: 2021
• Deposit Date: Oct 5, 2022
• Publicly Available Date: Oct 5, 2022
• Journal: Arkivoc
• Print ISSN: 1551-7004
• Electronic ISSN: 1551-7012
• Publisher: ARKAT USA
• Peer Reviewed: Peer Reviewed
• Volume: 2021
• Issue: 6
• Pages: 131 - 147
• DOI: https://doi.org/10.24820/ark.5550190.p011.589
• Public URL: https://durham-repository.worktribe.com/output/1190257