Peptoids with Antibiofilm Activity against the Gram Negative Obligate Anaerobe, Fusobacterium nucleatum

Toole, Jamie; Bolt, Hannah L.; Marley, John J.; Patrick, Sheila; Cobb, Steven L.; Lundy, Fionnuala T.

Abstract

Peptoids (oligo N-substituted glycines) are peptide analogues, which can be designed to mimic host antimicrobial peptides, with the advantage that they are resistant to proteolytic degradation. Few studies on the antimicrobial efficacy of peptoids have focused on Gram negative anaerobic microbes associated with clinical infections, which are commonly recalcitrant to antibiotic treatment. We therefore studied the cytotoxicity and antibiofilm activity of a family of peptoids against the Gram negative obligate anaerobe Fusobacterium nucleatum, which is associated with infections in the oral cavity. Two peptoids, peptoid 4 (NaeNpheNphe)4 and peptoid 9 (NahNspeNspe)3 were shown to be efficacious against F. nucleatum biofilms at a concentration of 1 μM. At this concentration, peptoids 4 and 9 were not cytotoxic to human erythrocytes or primary human gingival fibroblast cells. Peptoids 4 and 9 therefore have merit as future therapeutics for the treatment of oral infections.

Citation

Toole, J., Bolt, H. L., Marley, J. J., Patrick, S., Cobb, S. L., & Lundy, F. T. (2021). Peptoids with Antibiofilm Activity against the Gram Negative Obligate Anaerobe, Fusobacterium nucleatum. Molecules, 26(16), Article 4741. https://doi.org/10.3390/molecules26164741

• Journal Article Type: Article
• Acceptance Date: Aug 3, 2021
• Online Publication Date: Aug 5, 2021
• Publication Date: 2021
• Deposit Date: Aug 24, 2021
• Publicly Available Date: Aug 24, 2021
• Journal: Molecules
• Electronic ISSN: 1420-3049
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 26
• Issue: 16
• Article Number: 4741
• DOI: https://doi.org/10.3390/molecules26164741
• Public URL: https://durham-repository.worktribe.com/output/1236755