Kirsty Goncalves kirsty.e.goncalves@durham.ac.uk
Post Doctoral Research Associate
Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition
Goncalves, K.E.; Phillips, S.; Shah, D.S.H.; Athey, D.; Przyborski, S.A.
Authors
S. Phillips
D.S.H. Shah
D. Athey
Professor Stefan Przyborski stefan.przyborski@durham.ac.uk
Deputy Provost
Abstract
The microenvironment that cells experience during in vitro culture can often be far removed from the native environment they are exposed to in vivo. To recreate the physiological environment that developing neurites experience in vivo, we combine a well-established model of human neurite development with, functionalisation of both 2D and 3D growth substrates with specific extracellular matrix (ECM) derived motifs displayed on engineered scaffold proteins. Functionalisation of growth substrates provides biochemical signals more reminiscent of the in vivo environment and the combination of this technology with 3D cell culture techniques, further recapitulates the native cellular environment by providing a more physiologically relevant geometry for neurites to develop. This biomaterials approach was used to study interactions between the ECM and developing neurites, along with the identification of specific motifs able to enhance neuritogenesis within this model. Furthermore, this technology was employed to study the process of neurite inhibition that has a detrimental effect on neuronal connectivity following injury to the central nervous system (CNS). Growth substrates were functionalised with inhibitory peptides released from damaged myelin within the injured spinal cord (Nogo & OMgp). This model was then utilised to study the underlying molecular mechanisms that govern neurite inhibition in addition to potential mechanisms of recovery.
Citation
Goncalves, K., Phillips, S., Shah, D., Athey, D., & Przyborski, S. (2023). Application of biomimetic surfaces and 3D culture technology to study the role of extracellular matrix interactions in neurite outgrowth and inhibition. Biomaterials Advances, 144, Article 213204. https://doi.org/10.1016/j.bioadv.2022.213204
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 16, 2022 |
Online Publication Date | Nov 23, 2022 |
Publication Date | 2023-01 |
Deposit Date | Jan 9, 2023 |
Publicly Available Date | Jan 9, 2023 |
Journal | Biomaterials Advances |
Electronic ISSN | 2772-9508 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 144 |
Article Number | 213204 |
DOI | https://doi.org/10.1016/j.bioadv.2022.213204 |
Public URL | https://durham-repository.worktribe.com/output/1182096 |
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http://creativecommons.org/licenses/by/4.0/
Copyright Statement
Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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