Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III

Costello, Lydia; Goncalves, Kirsty; De Los Santos Gomez, Paola; Simpson, Amy; Maltman, Victoria; Ritchie, Pamela; Tasseff, Ryan; Isfort, Robert; Dicolandrea, Teresa; Wei, Xingtao; Määttä, Arto; Karakesisoglou, Iakowos; Markiewicz, Ewa; Bascom, Charles C.; Przyborski, Stefan

Abstract

Skin ageing is an intricate physiological process affected by intrinsic and extrinsic factors. There is a demand to understand how the skin changes with age and photoexposure in individuals with Fitzpatrick skin types I-III due to accelerated photoageing and the risk of cutaneous malignancies. To assess the structural impact of intrinsic and extrinsic ageing, we analysed 14 skin parameters from the photoprotected buttock and photoexposed dorsal forearm of young and ageing females with Fitzpatrick skin types II-III (n = 20) using histomorphic techniques. Whilst the minimum viable epidermis (Emin) remained constant (Q > 0.05), the maximum viable epidermis (Emax) was decreased by both age and photoexposure (Q ≤ 0.05), which suggests that differences in epidermal thickness are attributed to changes in the dermal-epidermal junction (DEJ). Changes in Emax were not affected by epidermal cell proliferation. For the first time, we investigated the basal keratinocyte morphology with age and photoexposure. Basal keratinocytes had an increased cell size, cellular height and a more columnar phenotype in photoexposed sites of young and ageing individuals (Q ≤ 0.05), however no significant differences were observed with age. Some of the most striking changes were observed in the DEJ, and a decrease in the interdigitation index was observed with both age and photoexposure (Q ≤ 0.001), accompanied by a decreased height of rête ridges and dermal papilla. Interestingly, young photoexposed skin was comparable to ageing skin across many parameters, and we hypothesise that this is due to accelerated photoageing. This study highlights the importance of skin care education and photoprotection from an early age.

Citation

Costello, L., Goncalves, K., De Los Santos Gomez, P., Simpson, A., Maltman, V., Ritchie, P., Tasseff, R., Isfort, R., Dicolandrea, T., Wei, X., Määttä, A., Karakesisoglou, I., Markiewicz, E., Bascom, C. C., & Przyborski, S. (2023). Quantitative morphometric analysis of intrinsic and extrinsic skin ageing in individuals with Fitzpatrick skin types II–III. Experimental Dermatology, 32(5), 620-631. https://doi.org/10.1111/exd.14754

• Journal Article Type: Article
• Acceptance Date: Jan 19, 2023
• Online Publication Date: Feb 3, 2023
• Publication Date: 2023-05
• Deposit Date: Feb 27, 2023
• Publicly Available Date: May 30, 2023
• Journal: Experimental Dermatology
• Print ISSN: 0906-6705
• Electronic ISSN: 1600-0625
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 32
• Issue: 5
• Pages: 620-631
• DOI: https://doi.org/10.1111/exd.14754
• Public URL: https://durham-repository.worktribe.com/output/1179663