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MERISTEM-DEFECTIVE regulates the balance between stemness and differentiation in the root meristem through RNA splicing control

Thompson, H.L.; Shen, W.; Matus, R.; Kakkar, M.; Jones, C.; Dolan, D.; Grellscheid, S.; Yang, X.; Zhang, N.; Mozaffari-Jovin, S.; Chen, C.; Zhang, X.; Topping, J.F.; Lindsey, K.

MERISTEM-DEFECTIVE regulates the balance between stemness and differentiation in the root meristem through RNA splicing control Thumbnail


Authors

W. Shen

R. Matus

M. Kakkar

C. Jones

X. Yang

N. Zhang

S. Mozaffari-Jovin

C. Chen

X. Zhang



Abstract

Plants respond to environmental stresses through controlled stem cell maintenance and meristem activity. One level of gene regulation is RNA alternative splicing. However, the mechanistic link between stress, meristem function and RNA splicing is poorly understood. The MERISTEM-DEFECTIVE (MDF) Arabidopsis gene encodes an SR-related family protein, required for meristem function and leaf vascularization, and is the likely orthologue of the human SART1 and yeast Snu66 splicing factors. MDF is required for the correct splicing and expression of key transcripts associated with root meristem function. We identified RSZ33 and ACC1, both known to regulate cell patterning, as splicing targets required for MDF function in the meristem. MDF expression is modulated by osmotic and cold stress, associated with differential splicing and specific isoform accumulation and shuttling between nucleus and cytosol, and acts in part via a splicing target SR34. We propose a model in which MDF controls splicing in the root meristem to promote stemness and to repress stress response, cell differentiation and cell death pathways.

Citation

Thompson, H., Shen, W., Matus, R., Kakkar, M., Jones, C., Dolan, D., …Lindsey, K. (2023). MERISTEM-DEFECTIVE regulates the balance between stemness and differentiation in the root meristem through RNA splicing control. Development, 150(7), Article dev201476. https://doi.org/10.1242/dev.201476

Journal Article Type Article
Acceptance Date Mar 13, 2023
Online Publication Date Apr 5, 2023
Publication Date 2023-04
Deposit Date Mar 14, 2023
Publicly Available Date Apr 11, 2023
Journal Development
Print ISSN 0950-1991
Electronic ISSN 1477-9129
Publisher The Company of Biologists
Peer Reviewed Peer Reviewed
Volume 150
Issue 7
Article Number dev201476
DOI https://doi.org/10.1242/dev.201476

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