Realtime wavefront sensing in a SPIM microscope, and active aberration tracking

Taylor, Jonathan M.; Saunter, Christopher D.; Bourgenot, Cyril; Girkin, John M.; Love, Gordon D.

Abstract

Adaptive optics (AO) can potentially allow high resolution imaging deep inside living tissue, mitigating against the loss of resolution due to aberrations caused by overlying tissue. Closed-loop AO correction is particularly attractive for moving tissue and spatially varying aberrations, but this requires direct wavefront sensing, which in turn requires suitable "guide stars" for use as wavefront references. We present a novel method for generating an orthogonally illuminated guide star suitable for direct wavefront sensing in a wide range of fluorescent biological structures, along with results demonstrating its use for measuring time-varying aberrations, in vivo.

Citation

Taylor, J. M., Saunter, C. D., Bourgenot, C., Girkin, J. M., & Love, G. D. (2015, February). Realtime wavefront sensing in a SPIM microscope, and active aberration tracking. Presented at Adaptive Optics and Wavefront Control for Biological Systems, San Francisco, CA

• Presentation Conference Type: Conference Paper (published)
• Conference Name: Adaptive Optics and Wavefront Control for Biological Systems
• Start Date: Feb 7, 2015
• End Date: Feb 12, 2015
• Online Publication Date: Mar 31, 2015
• Publication Date: 2015
• Deposit Date: Feb 13, 2018
• Publicly Available Date: Apr 22, 2022
• Volume: 9335
• Series ISSN: 1605-7422
• DOI: https://doi.org/10.1117/12.2080061
• Public URL: https://durham-repository.worktribe.com/output/1145869